qPCR Lentivirus Titer Kit

qPCR Lentivirus Titer Kit

Cat. No.	LV900
Unit	100 rxn

Cat. No.

LV900

Name

qPCR Lentivirus Titer Kit

Unit

100 rxn

The only kit on the market with complete elimination of NTC

abm’s qPCR Lentivirus Titer Kit is a one-step assay which employs a quick RNA extraction step that is followed by RT-qPCR. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits on the market. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.

Kit Features:

Saves time and eliminates inaccuracy: Quick RNA extraction
No NTC amplification: Eliminates non-specific (NTC) signals due to our unique primer design
Can be used directly on neat viral particles
Reduces variability: Ready-to-use reagent mix
RT-qPCR in one step: More sensitive and accurate than other methods
Contains dye comparable to SYBR Green™ and EvaGreen™

Product Component	Quantity
BlasTaq™ 2X qPCR Titer MasterMix	1.25 ml
Primer Mix	100 rxn (200 µl)
Standard Control DNA	50 µl
Virus Lysis Buffer	800 µl
ROX Reference Dye	15 µl
Nuclease-Free H₂O	2 x 1.0 ml

Customer Testimonials

"I recently received one of your qPCR Lentivirus Titration Kits and I think it is a wonderful product. The protocol is simple, fast, and accurate. I also really appreciate that there is an online titer calculator accompanying the kit on your website. The kit is allowing me to produce my own lentivirus, which is cheaper and more customizable."

- Kelly Taylor, Medical University of South Carolina

"We like the qPCR Lentivirus Titration(Titer) Kit. It is very easy to use and the titration can be completed in a short time."

- Ying Liu, University of Texas Health Science Center Houston

"We have been using Lentivirus qPCR kit to check our viruses for those viruses without reporter genes for a few years. The kit is very easy to use. We love it."

- Xinping Huang, Emory University

"I tested LV900 with the dilution that you suggested and also compared it to another method that measures the functional titer counting the viral integrations in the genome of HEK cells. Diluting the viral lysate solved my problems and improved my results. Now, I decided to use the kit for my future lentiviral titration. Thank you very much for your help and the great service!"

- Anna Schnaubelt, University Hospital Heidelberg

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Storage Condition

Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.

Note

qPCR Lentivirus Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

MACHINE TYPE	MACHINE
High ROX Machines	ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series Biometra TOptical Fluidigm BioMark™ Wafergene SmartChip System TianLong TL998 System
Low ROX Machines	ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7 BioGene InSyte™ Illumina Eco Analytikjena qTower Series Stratagene® Mx3000, Mx3005, Mx4000
No ROX machines	BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™ Roche LightCycler® (480, 1536, Nano) MJ Research Opticon™, Opticon™ 2, Chromo® 4 Eppendorf™ Realplex 4 BioGene SynChron™ Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600) Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat) Cepheid SmartCyler, GeneXpert Enigma™ ML Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS Qiagen Rotor-Gene™ (Q, 6000) Takara Dice™ Thermo Scientific PikoReal DNA-Technology DT96, DTlite, DT-322 Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680) Bioneer Exicycler™

Datasheet

Search CoA here

MSDS

LV900 MSDS

Other

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