Cell Immortalization Kits

Primary cells have a finite life span of less than 10-50 passages in vitro depending on cell type. Immortalizing your cell line will eliminate the frequent need to re-establish fresh cultures from specimens. Immortalized cell lines are capable of extended proliferation and should possess similar or identical genotype and phenotype to the parental tissue.

 

Immortalization Strategies for Mammalian Cells
Several methods exist for immortalizing mammalian cells in culture. With years of experience,abmhas developed the most comprehensive cell immortalization products comprising of ready-to-use retroviral, lentiviral and adenoviral vectors.

 

GENE OVEREXPRESSED VIRUSES OFFERED APPLICATION
hTERT Adenovirus,
Lentivirus,
Retrovirus
Reliable and commonly used.
SV40 T Antigen Adenovirus,
Lentivirus,
Retrovirus
Reliable and commonly used. Often used to immortalize epithelial cells.
EBV genes Epstein-Barr Virus Immortalize B lymphocytes, and sometimes T lymphocytes.
HPV E6-E7 Lentivirus Immortalize keratinocytes.
HOXB8, HOXA9, HOXA10 Lentivirus Immortalize hematopoietic cells, including macrophages, hematopoetic progenitor cells, and myeloid progenitor cells.
Adenoviral E1A-E1B Lentivirus Immortalize a wide range of primary rodent cells, including amniotic fluid cells, primary epithelial cells, fibroblasts, and primary kidney cells.
p53 siRNA, Rb siRNA Lentivirus Co-express with hTERT for better immortalization of epithelial cells.
Bmi1 Lentivirus Immortalize epithelial cells.
CDK4 Lentivirus Co-express with hTERT for a more authentic cell model.
Myc T58A (c-Myc), Ras V12 (H-Ras) Lentivirus Immortalizes a wide variety of cells, including neural stem cells, primary kidney cells, erythroblasts, and a variety of epithelial cells (primary prostate, mammary, airway, etc.).

Tools & Resources

Cell Immortalization Handbook

Cell Immortalization Handbook
This Handbook is designed to give an overview of Cell Immortalization, Including methods, protocols, and reagents.

Cell Immortalization Workflow

Cell Immortalization Workflow
Overview of abm‘s Immortalization service workflow. For more information, visit our Cell Immortalization Knowledge base.

Custom Cell Immortalization Service

Custom Cell Immortalization Service
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  1. Unit: 5 x 20 µl
    High Titer Lentivirus containing hTERT (109 IU/ml)
     

  2. Unit: 10 ml
    Lentivirus containing hTERT (106 IU/ml)
     

  3. Unit: 5 x 20 μl
    High Titer Lentivirus expressing SV40 large T antigen (109 IU/ml)
     

  4. Unit: 10 ml
    Lentivirus expressing SV40 large T antigen (106 IU/ml)
     

  5. Unit: 5 x 20 μl
    High Titer Lentivirus containing HPV-16 E6/E7 gene (109 IU/ml)
     

  6. Unit: 10 ml
    Lentivirus containing HPV-16 E6/E7 gene (106 IU/ml)
     

  7. High Titer Lentivirus expressing SV40 large T antigen (109 IU/ml)
     

  8. Unit: 10 ml
    Lentivirus expressing SV40 large T antigen (106 IU/ml)
     

  9. Unit: 5 x 20ul
    High Titer Recombinant CDK4 Lentivirus containing hTERT (109 IU/ml)
     

  10. Unit: 10ml
    Recombinant CDK4 Lentivirus containing hTERT (106 IU/ml)
     

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