pEU Vector

pEU vector is an expression vector that has been highly optimized to maximize synthetic efficiency through a wheat germ cell-free protein expression system.

By introducing bacteriophage SP6 into the transcriptional promoter and a completely artificial E01 sequence selected from a randomized nucleic acid sequence pool into the translational enhancer, an mRNA with high translational activity can be produced, and protein synthesis efficiency can be maximized. In addition to the vector outlined above, our company also offers vectors with added His-tag, GST-tag and DYKDDDDK-tag that are suited to affinity purification. You can choose which one you need depending on the situation.

Product lineup

Product Number	Product Name	Volume and Concentration	Size	Instruction manual	Safety Data Sheet
CFS-PEU-v1.0	pEU Vector set	10 µL each  [0.3 mg/ml]*	1 Set

* pEU-E01-DHFR (positive control) is 1.0 mg/mL.

Product Composition

Non-Tagged Vector

Product Name	Position of Tag		Vector Map	Sequence Data
pEU-E01-MCS	–	–

His-Tagged Vector

Product Name	Position of Tag	Protease Recognition Site	Vector Map	Sequence Data
pEU-E01-His-TEV-MCS-N1	N-terminus	TEV
pEU-E01-His-TEV-MCS-N2	N-terminus	TEV
pEU-E01-His-TEV-MCS-N3	N-terminus	TEV
pEU-E01-MCS-TEV-His-C1	C-terminus	TEV
pEU-E01-MCS-TEV-His-C2	C-terminus	TEV
pEU-E01-MCS-TEV-His-C3	C-terminus	TEV

GST-Tagged Vector

Product Name	Position of Tag	Protease Recognition Site	Vector Map	Sequence Data
pEU-E01-GST-PS-MCS-N1	N-terminus	PreScission
pEU-E01-GST-PS-MCS-N2	N-terminus	PreScission
pEU-E01-GST-PS-MCS-N3	N-terminus	PreScission
pEU-E01-MCS-PS-GST-C1	C-terminus	PreScission
pEU-E01-MCS-PS-GST-C2	C-terminus	PreScission

DYKDDDDK-Tagged Vector

Product Name	Position of Tag	Protease Recognition Site	Vector Map	Sequence Data
pEU-E01-DYKDDDDK-MCS-N1	N-terminus	–
pEU-E01-DYKDDDDK-MCS-N2	N-terminus	–
pEU-E01-DYKDDDDK-MCS-N3	N-terminus	–