pEU Vector

Dedicated expression vector that has been optimized for our wheat cell-free expression system

 

pEU Vector Series 

 

Porducts

pEU Vector

 

pEU vector is an expression vector that has been highly optimized to maximize synthetic efficiency through a wheat germ cell-free protein expression system.

 

By introducing bacteriophage SP6 into the transcriptional promoter and a completely artificial E01 sequence selected from a randomized nucleic acid sequence pool into the translational enhancer, an mRNA with high translational activity can be produced, and protein synthesis efficiency can be maximized. In addition to the vector outlined above, our company also offers vectors with added His-tag, GST-tag and DYKDDDDK-tag that are suited to affinity purification. You can choose which one you need depending on the situation.

Product lineup

 

Product Number Product Name Volume and Concentration Size Instruction manual Safety Data Sheet
CFS-PEU-v1.0 pEU Vector set  10 µL each  [0.3 mg/ml]*  1 Set btn download btn download

 

* pEU-E01-DHFR (positive control) is 1.0 mg/mL.

Product Composition

 
 
Non-Tagged Vector

 

Product Name Position of Tag   Vector Map Sequence Data
pEU-E01-MCS btn download btn download
 
 

 

His-Tagged Vector

 

Product Name Position of Tag Protease Recognition Site Vector Map Sequence Data
pEU-E01-His-TEV-MCS-N1 N-terminus TEV btn download btn download
pEU-E01-His-TEV-MCS-N2 N-terminus TEV btn download
pEU-E01-His-TEV-MCS-N3 N-terminus TEV btn download
pEU-E01-MCS-TEV-His-C1 C-terminus TEV btn download btn download
pEU-E01-MCS-TEV-His-C2 C-terminus TEV btn download
pEU-E01-MCS-TEV-His-C3 C-terminus TEV btn download
 
 

 

GST-Tagged Vector

 

Product Name Position of Tag Protease Recognition Site Vector Map Sequence Data
pEU-E01-GST-PS-MCS-N1 N-terminus PreScission btn download btn download
pEU-E01-GST-PS-MCS-N2 N-terminus PreScission btn download
pEU-E01-GST-PS-MCS-N3 N-terminus PreScission btn download
pEU-E01-MCS-PS-GST-C1 C-terminus PreScission btn download btn download
pEU-E01-MCS-PS-GST-C2 C-terminus PreScission btn download
 
 

 

DYKDDDDK-Tagged Vector

 

Product Name Position of Tag Protease Recognition Site Vector Map Sequence Data
pEU-E01-DYKDDDDK-MCS-N1 N-terminus btn download btn download
pEU-E01-DYKDDDDK-MCS-N2 N-terminus btn download
pEU-E01-DYKDDDDK-MCS-N3 N-terminus btn download