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Cell Immortalization Kits

Primary cells have a finite life span of less than 10-50 passages in vitro depending on cell type. Immortalizing your cell line will eliminate the frequent need to re-establish fresh cultures from specimens. Immortalized cell lines are capable of extended proliferation and should possess similar or identical genotype and phenotype to the parental tissue.

 

Immortalization Strategies for Mammalian Cells
Several methods exist for immortalizing mammalian cells in culture. With years of experience, abm has developed the most comprehensive cell immortalization products comprising of ready-to-use retroviral, lentiviral and adenoviral vectors.

 

All Lentiviruses listed below are provided at 109 IU/ml titer, 10 x 100 μl volume, and adenoviruses listed below are provided at 106 pfu/ml, 1 ml volume.

TERT Overexpression Viruses

May cause cell death in some cell types (eg. epithelial cells)

 

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Tools & Resources

 

Cell Immortalization Handbook
This Handbook is designed to give an overview of Cell Immortalization, Including methods, protocols, and reagents.

Cell Immortalization Workflow
Overview of abm’s Immortalization service workflow. For more information, visit our Cell Immortalization Knowledge base.

 

Custom Cell Immortalization Service
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