RNA Visualization

RNA Mango

 

The most advanced RNA tracking, visualization and pull down technology.

 

Technology for studying the diverse cellular roles of RNA has lagged behind the tools for studying DNA and proteins, but innovative researchers are working to change that! One such researcher is Dr. Peter Unrau of Simon Fraser University. He and his team have created RNA Mango, a novel technology with a number of useful applications.

 

Key Features

 

RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD ≈ 3nM) , and the strong ~1000X enhancement of the dye’s fluorescence when bound to the Mango aptamer (Fluorescent enhancement FE=1,100).

 

The TO dye has a number of other desirable properties including:

  • Small size
  • Lack of toxicity
  • Plasma and nuclear membrane permeability
  • Short intracellular half-life
  • The accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure

 

TO1-biotin is the standard variety of TO dye for RNA Mango experiments; other dye variants will also be available soon.

 

Can’t find what you’re looking for, or have a specific request?
Speak to one of our technical support specialists today technical@abmgood.com.

 

 

Product Details

The RNA Mango Aptamer

The RNA Mango Aptamer
The system has two components: the RNA Mango aptamer, and the TO-1 dye. The dye only fluoresces when bound to the Mango aptamer.

Tagging Your RNA

Tagging Your RNA
For mRNA inset the tag into the 3’UTR. For structured non-coding RNA replace a stem-loop that is not essential to the RNA function.

Application: RNA Visualization

Application: RNA Visualization
Express RNA of interest (with Mango Tag) using a vector or CRISPR Knock-In. Soak cells in TO-1 Biotin dye to illuminate RNA of interest localization

pApplication: RNA-RNA or RNA-protein complex pulldown

Application: RNA-RNA or RNA-protein complex pulldown
Express RNA of interest (with Mango Tag) using a vector or CRISPR Knock-In. Lyse the cells, and recover RNA of interest with bound RNA or proteins using streptavidin breads

Laboratory Results

Yeast U1 Ribonucleoprotein (RNP) Complex Pulldown Purification

Yeast U1 Ribonucleoprotein (RNP) Complex Pulldown Purification
Left: RNAs present in native extract in which U1M migrates as doublet. Right: U1M is a single band RNP present after mango-based purification using TO1 3PEG-Desthiobiotin Fluorophore

RNA Mango in a test tube

RNA Mango in a Test Tube
Mango dye binding to in vitro transcribed RNA Mango-tagged sgRNA. Stable binding and fluorescence even after leaving the tube for 1 month at room temperature.

 

Product UNIT CAT.NO.
TO1-3PEG-Biotin Fluorophore 0.5 mg/ml (500 µl) G955
TO1-3PEG-Desthiobiotin Fluorophore 0.5 mg/ml (500 µl) G956
TO3-3PEG-Biotin Fluorophore 0.5 mg/ml (500 µl) G959
YO3-3PEG-Biotin Fluorophore 0.5 mg/ml (500 µl) G957

Top Publications

Ribonucleoprotein purification and characterization using RNA Mango.

 

 

Panchapakesan SSS, Ferguson ML, Hayden EJ, Chen X, Hoskins AA, Unrau PJ. et al.
RNA. 2017 Oct;23(10):1592-1599.

 

DOI: 10.1261/rna.062166.117. PubMed: 28747322. PubMed Central PMCID: PMC5602116.

 

Structural basis for high-affinity fluorophore binding and activation by RNA Mango.

 

 

Trachman RJ 3rd, Demeshkina NA, Lau MWL, Panchapakesan SSS, Jeng SCY, Unrau PJ, Ferré-D’Amaré AR. et al.
Nat Chem Biol. 2017 Jul;13(7):807-813.

 

DOI: 10.1038/nchembio.2392. Epub 2017 May 29. PubMed : 28553947.PubMed Central PMCID: PMC5550021.

 

Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers.

 

 

Jeng SC, Chan HH, Booy EP, McKenna SA, Unrau PJ. et al
RNA. 2016 Dec;22(12):1884-1892. Epub 2016 Oct 24

 

PubMed : 27777365. PubMed Central PMCID: PMC5113208.

 

Browse Product

G955 TO1-3PEG-Biotin Fluorophore  0.5 mg/ml (500 µl)
G956 TO1-3PEG-Desthiobiotin Fluorophore  0.5 mg/ml (500 µl)
G957 YO3-3PEG-Biotin Fluorophore  0.5 mg/ml (500 µl)
G959 TO3-3PEG-Biotin Fluorophore  0.5 mg/ml (500 µl)