TO3-3PEG-Biotin Fluorophore

TO3-3PEG-Biotin Fluorophore

Cat. No.	G959
Unit	0.5 mg/ml (500 µl)

Cat. No.

G959

Name

TO3-3PEG-Biotin Fluorophore

Unit

0.5 mg/ml (500 µl)

Category

RNA Tracking (RNA Mango)

Description

RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD ≈ 3nM) , and the strong ~1000X enhancement of the dye’s fluorescence when bound to the Mango apatmer (Fluorescent enhancement FE=1,100).The TO dye has a number of other desirable properties including:

small size
lack of toxicity
plasma and nuclear membrane permeability
short intracellular half-life
the accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure
TO1 and TO3 dyes can be used in a 2-color reporter assay system using both the RNA Mango and RNA Peach Aptamer systems (Kong et. al. 2021)

Watch RNA Mango in Action

Transcription reaction were carried out in 300 µL volumes using T7 RNA polymerase (400 U, 50U/µL, applied biological materials), 0.5 µM TO1-3PEG-Biotin (applied biological materials), in 8 mM GTP, 5 mM CTP and ATP, 2 mM UTP, 40 mM TRIS buffer pH 7.9, 2.5 mM spermidine, 26 mM MgCl2, 20 mM KCl, Pyrophosphatase (0.5 U, 0.1 U/µL, ThermoFisher Scientific), and 0.01% Triton X-100. To each sample, either water (Negative), 0.33 µM DNA template (Mango Transcription), or 500 nM final Mango III A10U RNA (Positive) was added. Samples were visualized in a blue light box, movie is played back at 30X speed.

The most advanced RNA tracking, visualization and pull down technology.Technology for studying the diverse cellular roles of RNA has lagged behind the tools for studying DNA and proteins, but innovative researchers are working to change that! One such researcher is Dr. Peter Unrau of Simon Fraser University. He and his team have created RNA Mango, a novel technology with a number of useful applications.