PCR Polymerases
Meet the Next Generation of PCR Enzymes
abm is constantly evolving and so is our product line. Our pursuit of next generation PCR enzymes has led to intensive R&D and significant upgrades to our PCR products. The result – a new collection of world leading PCR enzymes and formulations with performance far beyond the current leading brands. Best of all – at the same discount prices we always offer.
Whether it’s for research or diagnostic product development, we are confident our premium enzymes will meet all your project needs. With abm, you will get wholesale discount pricing without the membership fees – what are you waiting for? Try our products today and take your PCR to the next level!
PCR Sure™ Kit
Simplify the PCR optimization process with our PCR Sure™ Kit. With a system consisting of multiple thermostable DNA polymerases pre-mixed with optimized buffers, your PCR is guaranteed to work!
Amplifies Difficult Template
Saves PCR Optimization Time
Guaranteed to Work
Super Versatile
Amplify your difficult templates with abm’s PCR Sure™ Kit. From crude templates to purified plasmids, High GC to Low GC, human, mouse, rat or plant DNA, one of our highly optimized mastermix is sure to work!
Extremely Easy to Use
With 8 optimized 2X MasterMixes, simply add your primers and template and run on one single program!
MegaFi™ Pro Fidelity DNA Polymerase
MegaFi™ Pro Fidelity DNA Polymerase is the best high fidelity DNA Polymerase on the market. With 2000X better fidelity than regular Taq, this ultrasensitive polymerase amplifies even the most difficult templates.
MegaFi™ Pro Fidelity
Amplifies difficult templates
Effective in long-range PCR
Ultra-Low Error Rates
MegaFi™ Pro DNA Polymerase is THE BEST high fidelity DNA polymerase A lacZa-containing sequence was amplified using various DNA polymerases, subcloned, and plated on X-gal-containing media. Colonies without PCR amplified lacZa mutations appear dark blue, while those that have PCR introduced frameshift mutations appear white.
Superior Fidelity
MegaFi™ DNA Polymerase has ~1300X higher fidelity than other enzymes on the market. abm’s MegaFi™ Fidelity DNA Polymerase (Cat. No. G896) has the highest accuracy rate compared to other DNA polymerases as tested using blue/white colony screening. Shown as relative fidelity compared to Taq DNA Polymerase (Taq = 1X). PCR cycle number and mutations resulting in non-phenotypic changes are accounted for.
Kodaq 2X PCR MasterMix
Kodaq 2X PCR MasterMix has a ready-to-use formula which includes a uniquely-formulated buffer with gel loading dye and strategically engineered mutations for robust, high-fidelity performance. The buffer accommodates high AT and GC content and common PCR inhibitors found in DNA samples.
Long Range PCR
Highly Robust
Superior sensitivity
Highly Robust
| PRODUCT | CAT.NO. |
|---|---|
| Kodaq 2X PCR MasterMix | G883 |
BlasTaq™ DNA Polymerase
Blast through a routine 2 hour PCR run in only 20 minutes with our BlasTaq™ DNA Polymerase. Reach extension speeds as fast as 6 kb/min and streamline your PCR!
6 kb/min extension speed
40-70% less time than Taq
Superior sensitivity
Super Fast Extension Speeds
Highly Robust
BlasTaq™ DNA Polymerase enables extension speeds as fast as 6 kb/min. Total reaction times for abm’s BlasTaq™ DNA Polymerase (Cat. No. G894) and Taq were determined for the amplification of different sized amplicons: 500 bp, 1 kb, 2 kb, and 5 kb. Reaction times are based on a 30-cycle program using the recommended reaction protocol for each enzyme.
PCR amplification using abm’s BlasTaq™ DNA Polymerase (Cat. No. G894) (column 1) of various targets vs. competitor polymerases (column 2 and 3) followed by electrophoresis on a 1% agarose gel. A) 1 kb target from Human cDNA. B) 1.3 kb target from plasmid DNA. C) 7.6 kb target from plasmid DNA.
| PRODUCT | CAT.NO. |
|---|---|
| BlasTaq™ DNA Polymerase | G894 |
| BlasTaq™ 2X PCR MasterMix | G895 |
| Ultra-Pure BlasTaq™ 2X PCR MasterMix | G885 |
| BlasTaq™ 2X qPCR MasterMix | G891, G892 |
| Ultra-Pure BlasTaq™ 2X qPCR MasterMix | G884 |
| BlasTaq™ Probe 2X qPCR MasterMix | G890 |
| BlasTaq™ Probe One-Step RT-qPCR * | G596 |
| *Contains OneScript™ Plus Reverse Transcriptase | G237 |
BlasTaq™ HotStart DNA Polymerase
Eliminate non-specific amplification and primer-dimer formation with our next generation BlasTaq™ HotStart DNA Polymerase. This formulation includes a proprietary antibody that blocks polymerase activity at low temperatures and dissociates at the denaturation step to restore enzyme activity.
No non-specific amplification or primer dimers
Improves yield of desired product
Simplifies reaction setup
Excellent for suspension cells
Super Fast Extension Speeds
BlasTaq™ HotStart DNA Polymerase eliminates non-specific amplification. abm ’s BlasTaq™ HotStart DNA Polymerase (Cat. No. G595) was used to amplify a 7 kb target. The antibody concentration was decreased incrementally from lanes 1-5. Lane 1 is our BlasTaq™ HotStart formulation and lane 5 contains no antibody.
BlasTaq™ HotStart DNA Polymerase has extension speeds of 6 kb/min. abm ’s BlasTaq™ HotStart DNA Polymerase (Cat. No. G595) was used to amplify different sized amplicons (500 bp, 1 kb, 2 kb, and 5 kb). Total reaction times are listed based on a 30-cycle program using the recommended reaction protocol for each enzyme.
| PRODUCT | CAT.NO. |
|---|---|
| BlasTaq™ HotStart DNA Polymerase | G595 |
BlasTaq™ HotStart DNA Polymerase
Eliminate non-specific amplification and primer-dimer formation with our next generation BlasTaq™ HotStart DNA Polymerase. This formulation includes a proprietary antibody that blocks polymerase activity at low temperatures and dissociates at the denaturation step to restore enzyme activity.
No non-specific amplification or primer dimers
Improves yield of desired product
Simplifies reaction setup
| PRODUCT | CAT.NO. |
|---|---|
BlasTaq™ HotStart DNA Polymerase |
G595 |
Eliminates Non-specific Amplification
BlasTaq™ HotStart DNA Polymerase eliminates non-specific amplification. abm ’s BlasTaq™ HotStart DNA Polymerase (Cat. No. G595) was used to amplify a 7 kb target. The antibody concentration was decreased incrementally from lanes 1-5. Lane 1 is our BlasTaq™ HotStart formulation and lane 5 contains no antibody.
Super Fast Extension Speeds
BlasTaq™ HotStart DNA Polymerase has extension speeds of 6 kb/min. abm ’s BlasTaq™ HotStart DNA Polymerase (Cat. No. G595) was used to amplify different sized amplicons (500 bp, 1 kb, 2 kb, and 5 kb). Total reaction times are listed based on a 30-cycle program using the recommended reaction protocol for each enzyme.
Unsure which polymerase is right for you?
Take your PCR to the next level with Ultra-Pure BlasTaq™ 2X PCR MasterMix. This formulation includes the same strategically-engineered, next generation Taq Polymerase which was subject to rigorous multi-step purification protocol using physical, chemical, and enzymatic methods for maximum removal of contaminating genomic DNA.
High purity
6 kb/min extension speed
40-70% less time than Taq
| PRODUCT | CAT.NO. |
|---|---|
Ultra-Pure BlasTaq™ 2X PCR MasterMix |
G885 |
High Purity
Ultra-Pure BlasTaq™ PCR MasterMix ensures efficient amplification while maintaining low-DNA contamination levels when compared to leading competitors. Two different primer sets (blue and orange) against host DNA were tested with two competitor products (1 and 2) and abm’s Ultra-Pure BlasTaq™ enzyme (3). There is no amplification seen in the no-template controls (NTC) from Ultra-Pure BlasTaq™ PCR MasterMix indicating exceptional purity of the product. Activity Control Primer Set demonstrated similar performance for all three tested products.
Unsure which polymerase is right for you?
Unsure which polymerase is right for your application? Contact us for assistance or use the simple PCR Enzyme Selection Guide below to compare the characteristics of each polymerase.
| CHARACTERISTIC | MegaFi™ Pro Fidelity | BlasTaq™ | BlasTaq™ HotStart | Ultra-Pure BlasTaq™ | Taq | Kodaq |
|---|---|---|---|---|---|---|
| Proofreading | Yes | No | No | No | No | Yes |
| Fidelity (vs. Native Taq) | 1300X | 1X | 1X | 1X | 1X | 50X |
| Specificity | •••• | ••• | •••• | ••• | ••• | •••• |
| Extension Speed | 20-30 sec/kb | 15 sec/kb | 15 sec/kb | 15 sec/kb | 60 sec/kb | 60 sec/kb |
| Max Target Length | 15-20 kb | 10-15 kb | 10-15 kb | 10-15 kb | 6.5 kb | 15-20 kb |
| Suitable for TA cloning | No | Yes | Yes | Yes | Yes | No |
| Special Feature | Fidelity and Sensitivity | Fast and Robust | High Specificity | High Purity | For routine PCR | Fidelity and Highly Robust |
Resources
Introduction to PCR
PCR Knowledge Base
Reverse Transcription
PCR Knowledge Base
