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Meet the Next Generation of PCR Enzymes

abm is constantly evolving and so is our product line. Our pursuit of next generation PCR enzymes has led to intensive R&D and significant upgrades to our PCR products. The result – a new collection of world leading PCR enzymes and formulations with performance far beyond the current leading brands. Best of all – at the same discount prices we always offer.

Whether it’s for research or diagnostic product development, we are confident our premium enzymes will meet all your project needs. With abm, you will get wholesale discount pricing without the membership fees – what are you waiting for? Try our products today and take your PCR to the next level!

 

 

Features:
 
  • Super Fast
  • Ultra Low Error Rates
  • Highly Robust
  • Wholesale Prices

MegaFi™ Fidelity DNA Polymerase


MegaFi™ Fidelity DNA Polymerase is the best high fidelity DNA Polymerase on the market. With 1300X better fidelity than regular Taq, this ultrasensitive polymerase amplifies even the most difficult templates.

1300X higher fidelity than Taq

Amplifies difficult templates

Effective in long-range PCR

Ultra-Low Error Rates

MegaFi™ DNA Polymerase is THE BEST high fidelity DNA polymerase. A lacZa-containing sequence was amplified using various DNA polymerases, subcloned, and plated on X-gal-containing media. Colonies without PCR amplified lacZa mutations appear dark blue, while those that have PCR introduced frameshift mutations appear white.

Superior Fidelity

MegaFi™ DNA Polymerase has ~1300X higher fidelity than other enzymes on the market. abm’s MegaFi™ Fidelity DNA Polymerase (Cat. No. G896) has the highest accuracy rate compared to other DNA polymerases as tested using blue/white colony screening. Shown as relative fidelity compared to Taq DNA Polymerase (Taq = 1X). PCR cycle number and mutations resulting in non-phenotypic changes are accounted for.

 
MegaFi™ Fidelity DNA Polymerase G896
MegaFi™ Fidelity 2X PCR MasterMix G897
MegaFi™ One-Step RT-PCR * G597
*Contains OneScript™ Hot Reverse Transcriptase (Cat. No. G593)

BlasTaq™ DNA Polymerase


Blast through a routine 2 hour PCR run in only 20 minutes with our BlasTaq™ DNA Polymerase. Reach extension speeds as fast as 6 kb/min and streamline your PCR!

6 kb/min extension speed

40-70% less time than Taq

Superior sensitivity


Super Fast Extension Speeds

BlasTaq™ DNA Polymerase enables extension speeds as fast as 6 kb/min. Total reaction times for abm’s BlasTaq™ DNA Polymerase (Cat. No. G894) and Taq were determined for the amplification of different sized amplicons: 500 bp, 1 kb, 2 kb, and 5 kb. Reaction times are based on a 30-cycle program using the recommended reaction protocol for each enzyme.

Highly Robust

 

PCR amplification using abm’s BlasTaq™ DNA Polymerase (Cat. No. G894) (column 1) of various targets vs. competitor polymerases (column 2 and 3) followed by electrophoresis on a 1% agarose gel. A) 1 kb target from Human cDNA. B) 1.3 kb target from plasmid DNA. C) 7.6 kb target from plasmid DNA.

BlasTaq™ DNA Polymerase G894
BlasTaq™ 2X PCR MasterMix G895
BlasTaq™ 2X qPCR MasterMix G891, G892
BlasTaq™ Probe 2X qPCR MasterMix G890
BlasTaq™ One-Step RT-qPCR* G598
BlasTaq™ Probe One-Step RT-qPCR * G596
*Contains OneScript™ Plus Reverse Transcriptase (Cat. No. G237)

BlasTaq™ HotStart DNA Polymerase


Eliminate non-specific amplification and primer-dimer formation with our next generation BlasTaq™ HotStart DNA Polymerase. This formulation includes a proprietary antibody that blocks polymerase activity at low temperatures and dissociates at the denaturation step to restore enzyme activity.

No non-specific amplification or primer dimers

Improves yield of desired product

Simplifies reaction setup


Eliminates Non-specific Amplification

BlasTaq™ HotStart DNA Polymerase eliminates non-specific amplification. abm ’s BlasTaq™ HotStart DNA Polymerase (Cat. No. G595) was used to amplify a 7 kb target. The antibody concentration was decreased incrementally from lanes 1-5. Lane 1 is our BlasTaq™ HotStart formulation and lane 5 contains no antibody.

BlasTaq™ HotStart DNA Polymerase G595

Super Fast Extension Speeds

BlasTaq™ HotStart DNA Polymerase has extension speeds of 6 kb/min. abm ’s BlasTaq™ HotStart DNA Polymerase (Cat. No. G595) was used to amplify different sized amplicons (500 bp, 1 kb, 2 kb, and 5 kb). Total reaction times are listed based on a 30-cycle program using the recommended reaction protocol for each enzyme.

Unsure which polymerase is right for you?

Unsure which polymerase is right for your application? Contact us for assistance or use the simple PCR Enzyme Selection Guide below to compare the characteristics of each polymerase.

 

CHARACTERISTIC MegaFi™ Fidelity BlasTaq™ BlasTaq™ HotStart
Proofreading Yes No No
Fidelity (vs. Native Taq) 1300X 1X 1X
Specificity •••• ••• ••••
Extension Speed 20-30 sec/kb 15 sec/kb 15 sec/kb
Target Length 15-20 kb 10-15 kb 10-15 kb
Suitable for TA cloning No Yes Yes
Special Feature Fidelity and Sensitivity Fast and Robust High Specificity

Resources

Introduction to PCR
Polymerase Chain Reaction Knowledge Base

DNA Polymerase Variations

DNA Polymerase Variation
Polymerase Chain Reaction Knowledge Base

Browse Product

MegaFi™ Fidelity DNA Polymerase G896
MegaFi™ Fidelity 2X PCR MasterMix G897
MegaFi™ One-Step RT-PCR * G597
*Contains OneScript™ Hot Reverse Transcriptase (Cat. No. G593)

 

BlasTaq™ DNA Polymerase G894
BlasTaq™ 2X PCR MasterMix G895
BlasTaq™ 2X qPCR MasterMix G891, G892
BlasTaq™ Probe 2X qPCR MasterMix G890
BlasTaq™ One-Step RT-qPCR* G598
BlasTaq™ Probe One-Step RT-qPCR * G596
*Contains OneScript™ Plus Reverse Transcriptase (Cat. No. G237)

 

BlasTaq™ HotStart DNA Polymerase G595

 

dNTP Mix

 

제품 번호 품   명    및    규    격
G050 dNTP Set            4 x 250 µl (100 mM each)
G010 dNTP Mix            250 µl, 10 mM each
G128 dNTP Mix            500 µl, 10 mM each
G129 dNTP Mix            1.0 ml, 10 mM each