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OneScript® Hot Reverse Transcriptase

Cat. No.
G593
Unit
100 rxn
Cat. No. G593
Name OneScript® Hot Reverse Transcriptase
Unit
100 rxn
Category PCR, qPCR, and RT
Description OneScript® Hot Reverse Transcriptase is a mutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase, that can reverse transcribe low abundance or degraded RNA, and has significantly better resistance to contaminating inhibitors such as reagents used during RNA extraction and contaminants from biological samples. High processivity and sensitivity allow for rapid cDNA synthesis of full-length cDNA fragments in a fraction of the time of leading competitors.

abm is the only company in the world to have a reverse transcriptase engineered to offer superior cDNA synthesis performance with even the most challenging RNA samples due to its incredible thermostability at 60-72ºC. This is the enzyme of choice for daily or demanding RNA reverse transcription. OneScript® Hot is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

Product Features:

  • Thermostable from 60-72°C/140-161°F
  • Transcribes degraded samples and resists inhibitors/contaminants
  • Provides high cDNA yields from difficult or ultra-low RNA samples
  • Earn 2X the rewards points

 

ISO 13485:2016 MDSAP Certified
Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

 

Product Component Quantity
OneScript® Hot Reverse Transcriptase 100 rxn (100 µl)
5X RT Buffer 400 µl
Storage Condition Store at -20°C.
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  • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
  • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
  • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).

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