T4 DNA Ligase
|
Cat. No.
|
G467 |
|---|---|
| Unit |
200 U (200 μl)
|
| Cat. No. | G467 |
| Name | T4 DNA Ligase |
| Unit | 200 U (200 μl) |
| Category | Molecular Biology Enzymes and Kits |
| Description | T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini. |
| Application | • Cloning of restriction fragments • Attaching double-stranded oligonucleotide linkers or adaptors to DNA • Site-directed mutagenesis • Nick repair of duplex DNA, RNA or DNA-RNA hybrids • Self-circularization of linear DNA |
| Concentration | 1 U/μl |
| Format General | Enzyme supplied with 5X Reaction Buffer |
| Storage Condition | Store all components at -20°C. |
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