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Reporter Cell Lines

abm’s reporter cell lines are the right tool for all research purposes as they are engineered to stably express reporters such as Luciferase, GFP, RFP, dual-reporter, and UTR. Find what you need and let abm be your one stop shop for all your research reagents and cell lines.

Can’t find what you are looking for? Contact us for more info.

 

Product Information

Characterization of BRITER cell line

Flow Cytometry Detection
Flow cytometry detection of cell surface recombinant CD2 from a HeLa cell line overexpressing stable tCD-Luc2 (T6023).

Reporter Cell Workflow

Reporter Cell Workflow
Click to see an overview of how we produce our Reporter Cell Lines.

Custom Stable Cell Line Generation Workflow

Custom Stable Cell Line Generation Service
abm provides a high quality and a cost-effective service for creating a cell line expressing your gene of interest with a fast turnaround time.

Top PubliCations

01

Inhibition of Lipid Oxidation Increases Glucose Metabolism and Enhances 2-Deoxy-2-[18F]Fluoro-d-Glucose Uptake in Prostate Cancer Mouse Xenografts.

Schlaepfer, I. R et al
Molecular Imaging and Biology 4:529-538 (2015).

DOI: 10.1007/s11307-014-0814-4.

02

Characterization of ryanodine receptor type 1 single channel activity using “on-nucleus” patch clamp.

Wagner, LE et al
Cell Calcium 56(2):96-107 (2014)

DOI: 10.1016/j.ceca.2014.05.004.

FAQs

  • How long can I store frozen vials for?

    Cells that are properly frozen using an effective cryoprotective agent can be stored in liquid nitrogen indefinitely without affecting their recovery.

  • Have had great results with expansion of these cells so far, we would like to do some in vitro assays to confirm bioluminescence in vitro, do you have a protocol for such an assay?

    We currently only provide a Luciferase assay kit that involves a cell lysis step. This product can be found here.

  • What type of GFP or RFP do these cell lines express, and from which promoter?

    The GFP is derived from copGFP (from Pontellina plumata) and the RFP is derived from mKate2 (from Entacmaea quadricolor). They are expressed from a proximal SV40 promoter, and a more distal CMV promoter may also drive expression. If you are looking for a cell line with a specific fluorescent reporter, or with a specific promoter, abm can perform a Stable Cell Line Generation service. Simply ask quotes@abmgood.com for a quote.

  • Is the GFP or RFP signal localized within the cell?

    No, there is no localization tag on the GFP or RFP, so they can diffuse throughout the cell. However, if you want a localized signal, abm's Organelle Labeling Lentiviruses can be used for our Stable Cell Line Generation service. Simply ask quotes@abmgood.com for a quote.

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Reporter

 

GFP 
RFP 

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Reporter Cells

 

 

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Reporters