0.5 mg/ml (500 µl)
|Unit||0.5 mg/ml (500 µl)|
|Category||RNA Tracking (RNA Mango)|
|Description||RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD ≈ 3nM) , and the strong ~1000X enhancement of the dye’s fluorescence when bound to the Mango apatmer (Fluorescent enhancement FE=1,100).The TO dye has a number of other desirable properties including:
TO1-biotin is the standard variety of TO dye for RNA Mango experiments; other dye variants will also be available soon.
Watch RNA Mango in Action
Transcription reaction were carried out in 300 µL volumes using T7 RNA polymerase (400 U, 50U/µL, applied biological materials), 0.5 µM TO1-3PEG-Biotin (applied biological materials), in 8 mM GTP, 5 mM CTP and ATP, 2 mM UTP, 40 mM TRIS buffer pH 7.9, 2.5 mM spermidine, 26 mM MgCl2, 20 mM KCl, Pyrophosphatase (0.5 U, 0.1 U/µL, ThermoFisher Scientific), and 0.01% Triton X-100. To each sample, either water (Negative), 0.33 µM DNA template (Mango Transcription), or 500 nM final Mango III A10U RNA (Positive) was added. Samples were visualized in a blue light box, movie is played back at 30X speed.
|Storage Condition||Stored at -20°C and protect from light.|
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- Abdolahzadeh, A., Dolgosheina, E. V., & Unrau, P. J. "RNA detection with high specificity and sensitivity using Nested Fluorogenic Mango NASBA" RNA rna-072629: (2019).
- Cawte, A. D., Unrau, P. J., & Rueda, D. S. "Live Cell Imaging of Single RNA Molecules with Fluorogenic Mango II Arrays" bioRxiv. : (2019). DOI: 10.1101/840082.
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