Reverse Transcription and RT-PCR

abm’s OneScript® and OneScript® Plus Reverse Transcriptases are engineered without intrinsic RNase H activity to increase cDNA synthesis yield and achievable length. OneScript® and OneScript® Plus’s exceptional sensitivity ensure high yields of full length cDNA even with only 0.1 pg of RNA template.

OneScript® can elongate up to 9 kb while OneScript® Plus’s extreme processivity enables it to elongate up to 15 kb. OneScript® Plus performs under high temperatures (45°C – 55°C), making it the better choice for cDNA synthesis from complex RNA templates (i.e. secondary structures and high GC content).

 

 

Product Details

OneScript® and OneScript® Plus are offered as independent enzymes in optimized buffers, cDNA Synthesis Kits, cDNA Synthesis SuperMixes, and a variety of other formats to suit many applications. The table below outlines the formats and their respective components:

 

FORMAT FEATURE COMPONENTS
Enzyme Only Suitable for any application
  • RTase and 5X RT Buffer
cDNA Synthesis Kit Flexible priming of oligo(dT) and random primers
  • RTase and 5X RT Buffer
  • Oligo(dT) and random primers
  • dNTPs
  • RNaseOFF Ribonuclease Inhibitor
cDNA Synthesis SuperMix Minimal pipetting steps
  • RTase
  • 2X Reaction Mix
All-In-One MasterMix Ultimate convenience and reproducibility
  • 5X All-In-One MasterMix
One-Step RT-PCR or RT-qPCR Kit One kit for downstream application
  • RTase
  • RT-PCR or RT-qPCR Enzyme Mix
  • PCR or qPCR MasterMix

 

Resources

Reverse Transcriptase Brochure

RT-PCR Product Brochure and Performance Data

Polymerase Chain Reaction (PCR) - Reverse Transcription Knowledge Base

Reverse Transcription (RT-PCR) Knowledge Base

PCR RT-PCR qPCR Application Handbook

PCR RT-PCR qPCR Application Handbook

Top Publications

MICU1 regulation of mitochondrial Ca(2+) uptake dictates survival and tissue regeneration

 

 

Antony, A., et al.
Nature Communications (2016) 9(7):10955-65.

 

Doi: 10.1038/ncomms10955

 

Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome

 

 

Wood, L. W., et al.
Scientific Reports (2016) 6(19857): 1-14.

 

Doi: 10.1038/srep19857

 

Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid binding protein

 

 

Ly, D., et al.
Scientific Reports (2016) 6(35651): 1-9.

 

Doi: 10.1038/srep35751

 

Browse Product

Reverse Transcriptases

 

G237      OneScript® Plus Reverse Transcriptase


 

One-Step BrightGreen qRT-PCR 

 

G471-S      One-Step BrightGreen qRT-PCR-No Dye

G471-R     One-Step BrightGreen qRT-PCR-ROX

G471-LR    One-Step BrightGreen qRT-PCR-Low ROX

G471-iC    One-Step BrightGreen qRT-PCR-iCycler


 

miRNA All-In-One cDNA Synthesis

 

G898      miRNA All-In-One cDNA Synthesis Kit

G899      miRNA All-In-One cDNA Synthesis Kit


 

 

NTP Set

 

제품 번호 품   명    및    규    격
G946 NTP Set                    4 x 250 µl (100 mM each)