OneScript® Hot cDNA Synthesis Kit

OneScript® Hot cDNA Synthesis Kit

Cat. No.	G594
Unit	100 rxn

Cat. No.

G594

Name

OneScript® Hot cDNA Synthesis Kit

Unit

100 rxn

Category

PCR, qPCR, and RT

Description

OneScript^® Hot cDNA Synthesis Kit is the first kit in the world that allows for efficient and robust cDNA synthesis at 60ºC. All other cDNA synthesis kits on the market are optimized for the reaction to be performed at 42-55ºC, which is sufficient for most RNA template, but fails on more difficult templates. abm is the only company in the world to have a reverse transcriptase engineered to offer superior cDNA synthesis performance with even the most challenging RNA samples due to its incredible thermostability at 60-72ºC.

The enzyme is a mutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase, that can reverse transcribe low abundance or degraded RNA, and has significantly better resistance to contaminating inhibitors such as reagents used during RNA extraction and contaminants from biological samples. High processivity and sensitivity allow for rapid cDNA synthesis of full-length cDNA fragments in a fraction of the time of leading competitors. OneScript^® Hot is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

Product Features:

Extremely thermostable cDNA synthesis at 60-72ºC
Transcribes degraded samples and resists inhibitors/contaminants
High cDNA yields from difficult or ultra-low RNA samples
Earn 2X the rewards points

ISO 13485:2016 MDSAP Certified
Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Product Component	Quantity
OneScript^® Hot Reverse Transcriptase	100 rxn (100 µl)
5X RT Buffer	400 µl
Oligo(dT) (10 μM)	100 µl
Random Primers (10 μM)	100 µl
dNTP (10 mM)	100 µl
Nuclease-Free H₂O	2 x 1.0 ml

Storage Condition

Store at -20°C.

Datasheet

G594 OneScript® Hot cDNA Synthesis Kit

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MSDS

G594 MSDS

Supporting Protocol

Other

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Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).

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