OneScript® Hot 5X RT MasterMix

OneScript® Hot 5X RT MasterMix

Cat. No.	G590
Unit	100 rxn (400 ul)

Specifications Datasheet Documents FAQs References Reviews

Cat. No.

G590

Name

OneScript® Hot 5X RT MasterMix

Unit

100 rxn (400 µl)

Category

Reverse Transcriptase & RT-PCR

Description

OneScript® Hot 5X RT MasterMix is a convenient, ready-to-use formulation designed for efficient first-strand cDNA synthesis. This product simplifies the cDNA synthesis process, ensuring reliability and reproducibility. The optimized 5X RT MasterMix contains abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a carefully balanced ratio of Oligo(dT)s and Random Primers. The resulting high-quality cDNA is suitable for a wide range of downstream applications.

Product Features:

Easy one-step setup reduces pipetting and sample handling
High cDNA yields from difficult samples with OneScript^® Hot Reverse Transcriptase (Cat. No. G593)
Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G591)
Also includes dNTPs, Oligo (dT)s, and random primers

Product Component	Quantity
OneScript® Hot 5X RT MasterMix	100 rxn (400 µl)
Nuclease-Free H₂O	1.0 ml

Note: This product is abm’s next generation of RT MasterMixes and is functionally equivalent to Cat. No. G485, G486, G490, G492, G590, G916 (5X All-In-One RT MasterMixes with AccuRT Genomic DNA Removal Kit or with ExCellenCT Lysis Kits), and G451, G452, G453, G454 (OneScript^® cDNA Synthesis SuperMixes), with improved performance. Contact our customer service team technical@abmgood.com for more information.

Application

Generation of templates for use in RT-PCR and qRT-PCR
cDNA synthesis from ssRNA
cDNA library construction
Generation of probes for hybridization
DNA primer extension

Concentration

5X

Storage Condition

Store at -20°C.

Note

If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592).

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G590

Controls and Related Product:

SafeView™ Classic