qPCR and One-Step RT MasterMixes
Enjoy World-Class Standards in Performance
Whether it’s for research or diagnostic applications, abm’s 15+ years of experience in PCR, qPCR, RT-qPCR, and RT-PCR product development have culminated in our world-class, specially formulated collection of MasterMixes and Kits.
All of our products are of premium quality and in the form of MasterMixes to save you time and streamline your experimental set-up. In addition, earn 2X the rewards points (2 points for every $1 ordered) when you order PCR products – exchange your points for coffee cards, movie tickets, vacation packages, and more. Visit our rewards program page for more details.
Features:
- Highly Sensitive
- Simple Workflows
- Fast Results
- Wholesale Prices
MasterMixes and Kits
Viral Titer Kits
Mycoplasma Detection
qPCR and One-Step RT MasterMixes
Streamline your qPCR, and RT-PCR with abm’s MasterMixes. Our specially formulated MasterMixes contain our next generation enzymes as well as all the ingredients you need premixed into an optimized format, significantly simplifying your workflow. Enjoy reduced handling errors, high reproducibility and excellent yields.
Premixed and ready-to-use
Simple and streamlined for high-throughput use
Reduce handling errors
Reliable Detection
Blastaq™ 2X qPCR MasterMix reliable quantifies low expression genes.Expression levels of 10 target genes in Human Colorectal Cancer Lines were quantified using Blastaq™ 2X qPCR MasterMix (Cat. No. G891). Values were normalized to GAPDH. Data courtesy of Dr. Vivek Vaish from Pune University.
Outstanding Thermostability
BlasTaq™ Probe One-Step RT-qPCR has superb sensitivity and signal-to-noise ratio. 20 µl One-Step RT-qPCR reactions (20 ng, 2 ng, 200 pg, 20 pg, or 2 pg of RNA per reaction) were carried out with abm’s BlasTaq™ Probe One-Step RT-qPCR (Cat. No. G596) for GAPDH amplification.
qPCR Virus Titer Kits
Get accurate titer results in only 1 hour with abm’s collection of viral titer kits for adeno-associated virus (AAV), lentivirus, and retrovirus. Simple, sensitive, and ready-to-use, abm’s viral titer kits contain our next generation PCR Polymerases or Reverse Transcriptases for the best enzyme performance on the market.
Results in only 1 hour
No non-specific (NTC) amplification
Ready-to-use, no purification step needed
Results in only 1 hour
abm’s qPCR Lentivirus Titer Kit provides results in only 1 hour. Total qRT-PCR reaction times of abm’s qPCR Lentivirus Titer Kit (Cat. No. LV900), Taqman® RNA-to-Ct™ 1-Step Kit, and time required for a typical 2-step reaction were compared.
Accurate Titers
abm’s qPCR AAV Titer Kit provides consistent, and accurate titer measurements. The titers of AAV3, 5, and 7 were measured using abm’s qPCR AAV Titer Kit (Cat. No. G931) containing primers for both CMV and ITR. The titer results for each serotype show there is no significant difference in real-time PCR amplification for CMV or ITR.
Easy Visualization
abm’s qPCR Lentivirus Titer Kit enables easy visualization of viral titers. A lentivirus sample (blue) was processed following the protocol for the qPCR Lentivirus Titer Kit (Cat. No. LV900) and amplified in a qPCR reaction along with standards included in the kit (purple).
Mycoplasma PCR Detection & Elimination Kit
Designed to perform with high specificity and sensitivity, abm’s Mycoplasma PCR Detection Kit minimizes false positives while ensuring coverage over 200 species/strains of Mycoplasma. The quick protocol makes routine screening simple and fast.
Get results in only 1 hr
Detects 200+ Mycoplasma species
Easy-to-use (no DNA isolation needed)
Sensitive Detection
abm’s Mycoplasma PCR Detection Kit can detect samples containing as low as 10 genome copies of Mycoplasma. abm‘s Mycoplasma PCR Detection Kit (Cat. No. G238) was used to detect samples containing ~10 to 100,000 genome copies of Mycoplasma. Presence of Mycoplasma was confirmed as a 450 bp band.
Effective Elimination
- Direct addition to cell culture
- Eliminate 70+ Mycoplasma species
- Results in 4-6 cell passages
- Non-toxic to most mammalian cells
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Tested on popular cell lines (e.g. HeLa)
Easy-to-use
Resources
Introduction to PCR
Polymerase Chain Reaction Knowledge Base
Reverse Transcription
Polymerase Chain Reaction Knowledge Base
