qPCR MasterMixes and Kits

Real-Time PCR is a PCR-based technique that is able to simultaneously amplify and detect changes in the amplicon concentration. Real-time PCR collects data during PCR amplification by utilizing fluorescence signal emitted by either DNA binding dyes such as SYBR Green Dye, or special probes such as TaqMan Probe.

 

 

Product Details

abm’s selection of BrightGreen qPCR MasterMix and TaqProbe qPCR MasterMix provides various options for both methods which also accommodates most qPCR thermal instrument models available on the market. The table below outlines our various qPCR MasterMixes and their recommended applications:

 

PRODUCT NAME SUITABLE APPLICATION
BrightGreen qPCR MasterMix
  • Dye-based, suitable for routine application
BrightGreen miRNA qPCR MasterMix
  • Dye-based, suitable for high-throughput screening
  • Required run time is 1/5 of standard protocol
BrightGreen KiloGreen qPCR MasterMix
  • Dye-based, suitable for small RNA
  • Recommended for miRNA studies
TaqProbe qPCR MasterMix
  • TaqMan-based, suitable for complex DNA fragments (i.e. high GC content and secondary structure)
  • Recommended for amplicon length of 300bp-1.3kb
One-Step RT-PCR or RT-qPCR Kit
  • Dye-based and TaqMan-based, suitable for routine application

 

Resources

qRT-PCR Brochure

qRT-PCR Product Brochure and Performance Data

Real-Time PCR/Quantitative PCR (qPCR) Knowledge Base

Real-Time PCR/Quantitative PCR (qPCR) Knowledge Base

PCR RT-PCR qPCR Application Handbook

PCR RT-PCR qPCR Application Handbook

Top Publications

MICU1 regulation of mitochondrial Ca(2+) uptake dictates survival and tissue regeneration

 

Antony, A., et al.
Nature Communications (2016) 9(7):10955-65.

 

Doi: 10.1038/ncomms10955

 

Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome

 

Wood, L. W., et al.
Scientific Reports (2016) 6(19857): 1-14.

 

Doi: 10.1038/srep19857

 

Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid binding protein

 

Ly, D., et al.
Scientific Reports (2016) 6(35651): 1-9.

 

Doi: 10.1038/srep35751

 

Browse Product


Blastaq™ Green 2X qPCR MasterMix

 

G891 Blastaq™ Green 2X qPCR MasterMix 4 x 1.25 ml, for 500 reactions (20 µl)
G892 Blastaq™ Green 2X qPCR MasterMix 25ml
G893 Blastaq™ Green 2X qPCR MasterMix 50.0ml

 


BrightGreen qPCR MasterMix

 

MasterMix-S BrightGreen 2X qPCR MasterMix-No Dye 4 x 1.25 ml, for 500 reactions (20 µl)
MasterMix-S-XL BrightGreen 2X qPCR MasterMix-No Dye 16 x 1.25 ml, for 2000 reactions (20 µl)
MasterMix-R BrightGreen 2X qPCR MasterMix-ROX 4 x 1.25 ml, for 500 reactions (20 µl)
MasterMix-R-XL BrightGreen 2X qPCR MasterMix-ROX 16 x 1.25 ml, for 2000 reactions (20 µl)
MasterMix-LR BrightGreen 2X qPCR MasterMix-Low ROX 4 x 1.25 ml for 500 reactions (20 µl)
MasterMix-LR-XL BrightGreen 2X qPCR MasterMix-Low ROX 16 x 1.25 ml, for 2000 reactions (20 µl)
MasterMix-iC BrightGreen 2X qPCR MasterMix-iCycler (5ml) 4 x 1.25 ml, for 500 reactions (20 µl)
MasterMix-iC-XL BrightGreen 2X qPCR MasterMix-iCycler (20ml) 16 x 1.25 ml, for 2000 reactions (20 µl)

 


 

BrightGreen Express 2X qPCR MasterMix

 

MasterMix-ES BrightGreen Express 2X qPCR MasterMix-No Dye 4 x 1.25 ml, for 500 reactions (20 µl)
MasterMix-ER BrightGreen Express 2X qPCR MasterMix-ROX 4 x 1.25 ml, for 500 reactions (20 µl)
MasterMix-EL BrightGreen Express 2X qPCR MasterMix-Low ROX 4 x 1.25 ml, for 500 reactions (20 µl)
MasterMix-EC BrightGreen Express 2X qPCR MasterMix-iCycler 4 x 1.25 ml, for 500 reactions (20 µl)

 


 

BrightGreen miRNA qPCR MasterMix

 

MasterMix-mS BrightGreen miRNA qPCR MasterMix-No Dye 4 x 1.25 ml for 500 reactions
MasterMix-mR BrightGreen miRNA qPCR MasterMix-ROX 4 x 1.25 ml for 500 reactions
MasterMix-mL BrightGreen miRNA qPCR MasterMix-Low ROX 4 x 1.25 ml for 500 reactions
MasterMix-mC BrightGreen miRNA qPCR MasterMix-iCycler 4 x 1.25 ml for 500 reactions

 


 

One-Step BrightGreen qRT-PCR

 

G471-S One-Step BrightGreen qRT-PCR-No Dye 100 rxn (20 µl/rxn)
G471-R One-Step BrightGreen qRT-PCR-ROX 100 rxn (20 µl/rxn)
G471-LR One-Step BrightGreen qRT-PCR-Low ROX 100 rxn (20 µl/rxn)
G471-iC One-Step BrightGreen qRT-PCR-iCycler 100 rxn (20 µl/rxn)

 


 


 

qPCR Lentivirus Titration(Titer) Kit

 

LV900 qPCR Lentivirus Titration(Titer) Kit 100 Reactions