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qPCR and One-Step RT MasterMixes

Enjoy World-class Standards in Performance 

 

Whether it’s for research or diagnostic applications, abm’s 15+ years of experience in PCR, qPCR, RT-qPCR, and RT-PCR product development have culminated in our world-class, specially formulated collection of MasterMixes and Kits.

 

 

Features:

 

 
  • Highly Sensitive
  • Simple Workflows
  • Fast Results
  • Wholesale Prices

 

MasterMixes and Kits

 

Viral Titer Kits

 

Mycoplasma Detection

qPCR and One-Step RT MasterMixes


Streamline your qPCR, and RT-PCR with abm’s MasterMixes. Our specially formulated MasterMixes contain our next generation enzymes as well as all the ingredients you need premixed into an optimized format, significantly simplifying your workflow. Enjoy reduced handling errors, high reproducibility and excellent yields.

Premixed and ready-to-use

Simple and streamlined for high-throughput use

Reduce handling errors

Dye-based qPCR

BlasTaq™ 2X qPCR MasterMix G891,G892

Outstanding Thermostability

 

BlasTaq™ Probe One-Step RT-qPCR has superb sensitivity and signal-to-noise ratio. 20 µl One-Step RT-qPCR reactions (20 ng, 2 ng, 200 pg, 20 pg, or 2 pg of RNA per reaction) were carried out with abm’s BlasTaq™ One-Step RT-qPCR (Cat. No. G598) for GAPDH amplification.

Probe-based qPCR

BlasTaq™ Probe 2X qPCR MasterMix G890

 

One-Step RT

BlasTaq™ One-Step RT-qPCR G598
BlasTaq™ Probe One-Step RT-qPCR G596
MegaFi™ One-Step RT-PCR* G597
* Contains OneScript™ Hot Reverse Transcriptase (Cat. No. G593)

Viral Titer Kits


Get accurate titer results in only 1 hour with abm’s collection of viral titer kits for adeno-associated virus (AAV), lentivirus, and retrovirus. Simple, sensitive, and ready-to-use, abm’s viral titer kits contain our next generation PCR Polymerases or Reverse Transcriptases for the best enzyme performance on the market.

Results in only 1 hour

No non-specific (NTC) amplification

Ready-to-use, no purification step needed

qPCR AAV Titer Kit G931
qPCR Lentivirus Titer Kit LV900
qPCR Retrovirus Titer Kit G949

Results in only 1 hour

 

abm’s qPCR Lentivirus Titer Kit provides results in only 1 hour. Total qRT-PCR reaction times of abm’s qPCR Lentivirus Titer Kit (Cat. No. LV900), Taqman® RNA-to-Ct™ 1-Step Kit, and time required for a typical 2-step reaction were compared.

Accurate Titers

 

abm’s qPCR AAV Titer Kit provides consistent, and accurate titer measurements. The titers of AAV3, 5, and 7 were measured using abm’s qPCR AAV Titer Kit (Cat. No. G931) containing primers for both CMV and ITR. The titer results for each serotype show there is no significant difference in real-time PCR amplification for CMV or ITR.

Mycoplasma PCR Detection Kit


Designed to perform with high specificity and sensitivity, abm’s Mycoplasma PCR Detection Kit minimizes false positives while ensuring coverage over 200 species/strains of Mycoplasma. The quick protocol makes routine screening simple and fast.

Get results in only 1 hr

Detects 200+ Mycoplasma species

Easy-to-use (no DNA isolation needed)

Mycoplasma PCR Detection Kit G238
Mycoplasma PCR Detection & Elimination Combo Kit G238-G398

Sensitive Detection

 

abm’s Mycoplasma PCR Detection Kit can detect samples containing as low as 10 genome copies of Mycoplasma. abm‘s Mycoplasma PCR Detection Kit (Cat. No. G238) was used to detect samples containing ~10 to 100,000 genome copies of Mycoplasma. Presence of Mycoplasma was confirmed as a 450 bp band.

Resources

 

Introduction to PCR

Polymerase Chain Reaction Knowledge Base

 

Reverse Transcription
Polymerase Chain Reaction Knowledge Base