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Cat. No.
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E017 |
|---|---|
| Unit |
100 U (100 μl)
|
| Cat. No. | E017 |
| Name | Poly(A) Polymerase, Yeast |
| Unit |
100 U (100 μl)
|
| Category | Molecular Biology Enzymes and Kits |
| Description | Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli. |
| Application | • Labelling of RNA with ATP or cordycepin • Poly(A) tailing of RNA for cloning or affinity purification • Increasing translation of RNA transferred into eukaryotic cells |
| Concentration | 1 U/μl |
| Components | Enzyme supplied with 5X Reaction Buffer |
| Storage Buffer | 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol. |
| Storage Condition | Store all components at -20°C. |
| Note | One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C. |
| Caution | This product is distributed for laboratory research only. Not for diagnostic use . |
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- Alghamdi, T.. "Insights into Glomerular Cell Biology in Health and Disease (Doctoral dissertation)" University of Toronto Toronto:Canada (2019).
- Hou, W., Singh, R. K., Zhao, P., Martins, V., Aguilar, E., Canto, T., ... & Dias, A. C. P. "Transgenic expression of Hyp-1 gene from Hypericum perforatum L" alters expression of defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens. Planta 251(1):13 (2020).
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