페이지 선택

Poly(A) Polymerase, Yeast

Cat. No.
E017
Unit
100 U (100 μl)
Cat. No. E017
Name Poly(A) Polymerase, Yeast
Unit
100 U (100 μl)
Category Molecular Biology Enzymes and Kits
Description Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.
Application • Labelling of RNA with ATP or cordycepin
• Poly(A) tailing of RNA for cloning or affinity purification
• Increasing translation of RNA transferred into eukaryotic cells
Concentration 1 U/μl
Components Enzyme supplied with 5X Reaction Buffer
Storage Buffer 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
Storage Condition Store all components at -20°C.
Note One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.
Caution This product is distributed for laboratory research only. Not for diagnostic use .
Search CoA here
MSDS

There are no FAQs for this product yet!

  • Alghamdi, T.. "Insights into Glomerular Cell Biology in Health and Disease (Doctoral dissertation)" University of Toronto Toronto:Canada (2019).
  • Hou, W., Singh, R. K., Zhao, P., Martins, V., Aguilar, E., Canto, T., ... & Dias, A. C. P. "Transgenic expression of Hyp-1 gene from Hypericum perforatum L" alters expression of defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens. Planta 251(1):13 (2020).

This product has no review yet.

Controls and Related Product:

Poly(A) Polymerase, Yeast


Cat. No.
E017
Unit
100 U (100 μl)

Column-Pure RNA Miniprep Kit


Cat. No.
D518
Unit 50

T4 DNA Ligase


Cat. No.
G467
Unit 200 U (200 μl)

Column-Pure Gel and PCR Clean-Up Kit


Cat. No.
D516
Unit 100

RNase R


Cat. No.
E049
Unit 500 U (50 μl)

Column-Pure Plasmid Mini-Prep Kit


Cat. No.
D514
Unit 100

Pro Ligation-Free Cloning Kit (20 reactions)


Cat. No.
E086
Unit 20 Reactions

OneScribe T7 Transcription Kit


Cat. No.
E081
Unit 50 Reactions