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All-In-One 5X RT MasterMix

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CAT.NO UNIT
G592 100  rxn

Specifications

Description

High cDNA yields at affordable prices!

All-In-One 5X RT MasterMix is a convenient and ready-to-use formulation for first-strand cDNA synthesis, including genomic DNA (gDNA) removal. Genomic DNA contamination is a common problem for accurate RNA detection and this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will be simple, reliable, and reproducible. The optimized 5X RT MasterMix contains abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a finely-balanced ratio of Oligo (dT)s and Random Primers. The high-quality cDNA synthesized from this kit can be used for a wide range of downstream applications.

Product Features:

  • Easy One-Step setup reduces pipetting and sample handling
  • High cDNA yields from difficult samples with OneScript® Hot Reverse Transcriptase (Cat. No. G593)
  • Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G591)
  • Removes contaminating gDNA with temperature-sensitive DNase
  • Also includes dNTPs, Oligo (dT)s, and random primers
SKU G592
Concentration 5X
Storage Condition

Store at -20°C.
Unit quantity 100 rxn

OneScript® Hot Reverse Transcriptase

Synthesize cDNA from complex RNA templates with abm’s OneScript® Hot Reverse Transcriptase. Engineered for outstanding thermostability, our OneScript® Hot Reverse Transcriptase is ideal for handling secondary structures (e.g. GC-rich templates) as well as long, degraded, or contaminated RNA samples, making it the superior choice for challenging RNA templates.

 

Extreme thermostability At 50-80°C

Transcribes degraded samples and resists inhibitors/contaminants

High cDNA yields from difficult or ultra-low RNA samples

OneScript® Hot Reverse Transcriptase G593
OneScript® Hot cDNA Synthesis Kit G594
MegaFi™ One-Step RT-PCR G597
All-in-One 5X RT MasterMix G592

Outstanding Thermostability

OneScript® Hot Reverse Transcriptase has exceptional thermostability. OneScript® Hot Reverse Transcriptase (Cat. No. G593) was used in a 10 min. reaction with RNA template at temperatures ranging from 50 and 80˚C. The resulting synthesized cDNA was followed by PCR and visualized on a 1% agarose gel.

Documents

Supporting Protocol

 

 

 

MSDS

 

 

FAQs

References

  • Lee, G et al. “Potential monoclonal antibody therapy for the treatment of ovarian cancer” Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.

 

  • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. “PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε” Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.

 

  • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. “Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress” Metallomics. : (2019).