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MegaFi™ One-Step RT-PCR

CAT.NO UNIT
G597 100 rxn

Specifications

Description

Simple One Step Reaction, Superior Performance

 

MegaFiTM One-Step RT-PCR is an enzyme mix of OneScript® Hot Reverse Transcriptase and MegaFiTM Fidelity DNA Polymerase with RNaseOFF Ribonuclease Inhibitor, gel loading dye, and all other necessary reagents in a single One-Step 2X RT-PCR Buffer for both highly sensitive and specific reverse transcription and high fidelity PCR amplification in a single reaction tube from any RNA template. It provides flexibility in choosing desired primers for use with a proprietary RT-PCR buffer containing stabilizers and enhancers that optimize the two reactions in a “single step”. This kit offers simple, efficient reaction setup, and is a reliable alternative to conventional “two-step” sequential RT-PCR.

Product Features:

  • Enjoy simple, one-step RT-PCR reactions
  • 1300X lower error rates due to our MegaFi™ Fidelity DNA Polymerase (Cat. No. G896)
  • Extremely thermostable cDNA synthesis at 50-80°C due to our OneScript® Hot Reverse Transcriptase (Cat. No. G593)
  • Excellent protection against common ribonucleases due to our RNaseOFF Ribonuclease Ihibitor (Cat. No. G591)
SKU G597
Storage Condition

Store at -20°C.
Unit quantity 100 rxn

MegaFi™ Fidelity DNA Polymerase

MegaFi™ Fidelity DNA Polymerase is the best high fidelity DNA Polymerase on the market. With 1300X better fidelity than regular Taq, this ultrasensitive polymerase amplifies even the most difficult templates.

1300X higher fidelity than Taq

Amplifies difficult templates

Effective in long-range PCR

Ultra-Low Error Rates

MegaFi™ DNA Polymerase is THE BEST high fidelity DNA polymerase. A lacZa-containing sequence was amplified using various DNA polymerases, subcloned, and plated on X-gal-containing media. Colonies without PCR amplified lacZa mutations appear dark blue, while those that have PCR introduced frameshift mutations appear white.

Superior Fidelity

MegaFi™ DNA Polymerase has ~1300X higher fidelity than other enzymes on the market. abm’s MegaFi™ Fidelity DNA Polymerase (Cat. No. G896) has the highest accuracy rate compared to other DNA polymerases as tested using blue/white colony screening. Shown as relative fidelity compared to Taq DNA Polymerase (Taq = 1X). PCR cycle number and mutations resulting in non-phenotypic changes are accounted for.

 

OneScript® Hot Reverse Transcriptase

Synthesize cDNA from complex RNA templates with abm’s OneScript® Hot Reverse Transcriptase. Engineered for outstanding thermostability, our OneScript® Hot Reverse Transcriptase is ideal for handling secondary structures (e.g. GC-rich templates) as well as long, degraded, or contaminated RNA samples, making it the superior choice for challenging RNA templates.

 

Extreme thermostability At 50-80°C

Transcribes degraded samples and resists inhibitors/contaminants

High cDNA yields from difficult or ultra-low RNA samples

Outstanding Thermostability

OneScript® Hot Reverse Transcriptase has exceptional thermostability. OneScript® Hot Reverse Transcriptase (Cat. No. G593) was used in a 10 min. reaction with RNA template at temperatures ranging from 50 and 80˚C. The resulting synthesized cDNA was followed by PCR and visualized on a 1% agarose gel.

FAQs

References

  • Lee, G et al. “Potential monoclonal antibody therapy for the treatment of ovarian cancer” Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.

 

 

  • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. “PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε” Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.

 

  • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. “Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress” Metallomics. : (2019).