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Cat. No.
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E091 |
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| Unit |
200 U (100 μl)
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| Cat. No. | E091 | ||||||
| Name | DNase I (RNase-Free) | ||||||
| Unit |
200 U (100 μl)
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| Category | Molecular Biology Enzymes and Kits | ||||||
| Description | DNase I is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. This product is RNase free and can be used in RNA applications.
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| Application | • Removes DNA from protein preparations and RNA samples • Mediates nick translation • Generates random fragments for dideoxy sequencing • Degrade template DNA following in vitro transcription • Mediate DNase I foot-printing |
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| Concentration | 2 U/μl | ||||||
| Note | One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.
This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C. For non-RNAse-Free DNAse I, see Cat. No. G028. This product is distributed for laboratory research only Caution: Not for diagnostic use. |
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