Tn5 Transposase for Tagmentation
Cat. No.
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E094 |
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Unit | 100 rxn |
Cat. No. | E094 | ||||||||||
Name | Tn5 Transposase for Tagmentation | ||||||||||
Unit |
100 rxn
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Category | PCR, qPCR, and RT | ||||||||||
Description | abm’s Tn5 Transposase for Tagmentation is comprised of our hyperactive Protein A-tagged Tn5 transposase for use in generating Next Generation Sequencing (NGS) libraries. This enzyme has the unique ability to fragment DNA (genomic DNA, plasmid DNA, and PCR amplicons) and add Illumina-compatible sequencing adaptors. The technology shows a high rate of reproducibility, requires low DNA input, and can be applied for various research applications, including stem cells, tumors, epigenetics, etc.
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Reaction Definition | 1X Universal Reaction Enzyme Reaction buffer (Cat. E204) and incubate at 37°C. | ||||||||||
Note | Reaction Buffer Compatibility
Buffer 1: 100%, Buffer 2: 100%, Buffer 3: 75%, Universal Buffer: 100% Heat inactivation 65°C for 20 minutes. |
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