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Tn5 Transposase for Tagmentation

Cat. No.
E094
Unit 100 rxn
Cat. No. E094
Name Tn5 Transposase for Tagmentation
Unit
100 rxn
Category PCR, qPCR, and RT
Description abm’s Tn5 Transposase for Tagmentation is comprised of our hyperactive Protein A-tagged Tn5 transposase for use in generating Next Generation Sequencing (NGS) libraries. This enzyme has the unique ability to fragment DNA (genomic DNA, plasmid DNA, and PCR amplicons) and add Illumina-compatible sequencing adaptors. The technology shows a high rate of reproducibility, requires low DNA input, and can be applied for various research applications, including stem cells, tumors, epigenetics, etc.

 

Product Component Quantity
Hyperactive pA-Tn5 Transposase (500ng/µl) 60µl (100 rxn)
Annealing Buffer 1.25ml
Coupling Buffer 200µl
5X Tagmentation Buffer 500µl
Reaction Definition 1X Universal Reaction Enzyme Reaction buffer (Cat. E204) and incubate at 37°C.
Note Reaction Buffer Compatibility

Buffer 1: 100%, Buffer 2: 100%, Buffer 3: 75%, Universal Buffer: 100%

Heat inactivation

65°C for 20 minutes.

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