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T7 RNA Polymerase
T7 RNA Polymerase
| CAT.NO | UNIT |
|---|---|
| E041 | 100 ul (5000 U) |
| Cat. No. | E041 |
| Name | T7 RNA Polymerase |
| Unit |
100 ul (5000 U)
|
| Category | Molecular Biology Enzymes and Kits |
| Description | T7 RNA Polymerase is a DNA dependent RNA polymerase that catalyzes the synthesis of RNA in the 5´→ 3´ direction only in the presence of its cognate T7 phage promoter sequence. T7 RNA Polymerase has high specificity for the T7 phage promoter and will not recognize SP6 or T3 RNA Polymerase promoter sequences. |
| Application | • Synthesis of RNA transcripts for hybridization probes
• Synthesis of RNA for in vitro translation • Synthesis of biologically active mRNA • Generate large amounts of labelled or non-labelled RNA |
| Concentration | 50 U/μl |
| Storage Buffer | 50 mM Tris-HCl (pH 8), 1 mM EDTA, 20 mM β-ME, 100 mM NaCl, 0.1% Triton®X-100 and 50% (v/v) Glycerol. |
| Storage Condition | Store all components at -20°C. |
| Note | One unit is defined as the amount of T7 RNA Polymerase that is required to incorporate 1 nmol ATP into acid-insoluble material in a 50 μl reaction volume in 1 hour at 37°C in 1X T7 RNA Polymerase Reaction Buffer. |
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- Abdolahzadeh, A., Dolgosheina, E. V., & Unrau, P. J. "RNA detection with high specificity and sensitivity using Nested Fluorogenic Mango NASBA" RNA rna-072629: (2019).
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