페이지 선택

T7 RNA Polymerase

CAT.NO UNIT
E041 100 ul (5000 U)
Cat. No. E041
Name T7 RNA Polymerase
Unit
100 ul (5000 U)
Category Molecular Biology Enzymes and Kits
Description T7 RNA Polymerase is a DNA dependent RNA polymerase that catalyzes the synthesis of RNA in the 5´→ 3´ direction only in the presence of its cognate T7 phage promoter sequence. T7 RNA Polymerase has high specificity for the T7 phage promoter and will not recognize SP6 or T3 RNA Polymerase promoter sequences.
Application • Synthesis of RNA transcripts for hybridization probes

• Synthesis of RNA for in vitro translation

• Synthesis of biologically active mRNA

• Generate large amounts of labelled or non-labelled RNA

Concentration 50 U/μl
Storage Buffer 50 mM Tris-HCl (pH 8), 1 mM EDTA, 20 mM β-ME, 100 mM NaCl, 0.1% Triton®X-100 and 50% (v/v) Glycerol.
Storage Condition Store all components at -20°C.
Note One unit is defined as the amount of T7 RNA Polymerase that is required to incorporate 1 nmol ATP into acid-insoluble material in a 50 μl reaction volume in 1 hour at 37°C in 1X T7 RNA Polymerase Reaction Buffer.
Search CoA here
There are no FAQs for this product yet!
  • Abdolahzadeh, A., Dolgosheina, E. V., & Unrau, P. J. "RNA detection with high specificity and sensitivity using Nested Fluorogenic Mango NASBA" RNA rna-072629: (2019).

This product has no review yet.

Controls and Related Product:

Poly(A) Polymerase, Yeast


Cat. No.
E017
Unit
100 U (100 μl)

Column-Pure RNA Miniprep Kit


Cat. No.
D518
Unit 50

T4 DNA Ligase


Cat. No.
G467
Unit 200 U (200 μl)

Column-Pure Gel and PCR Clean-Up Kit


Cat. No.
D516
Unit 100

RNase R


Cat. No.
E049
Unit 500 U (50 μl)

Column-Pure Plasmid Mini-Prep Kit


Cat. No.
D514
Unit 100

Pro Ligation-Free Cloning Kit (20 reactions)


Cat. No.
E086
Unit 20 Reactions

OneScribe T7 Transcription Kit


Cat. No.
E081
Unit 50 Reactions