abm's Mycoplasma PCR Detection Kit (Cat. No. G238) offers highly specific and sensitive detection of 200+ strains of Mycoplasmas in less than 2 hours. This bestselling kit is designed to minimize false positives, ensuring quick and reliable routine screening of cell cultures. A recent official publication by a national public health agency found that our kit out-performs Sigma and Lonza's kits while being 6X less expensive than ATCC's kit (Russell et al., 2020).

Mycoplasmas are highly undesirable, easily acquired, and notoriously difficult to detect, altering infected cells at the molecular level and leading to visible changes in cell morphology and growth characteristics. Timely detection of Mycoplasmas in cell cultures is recommended in order to deter wide-spread contamination and save on the costly efforts of elimination. Our proprietary MasterMix formulation contains a gel loading dye, making it convenient for gel electrophoresis.

Kit Features:

• No DNA isolation needed: Directly add cell culture supernatants to PCR reaction
• Rapid and Comprehensive: Detect 200+ Mycoplasma species in less than 2 hours
• Highly Sensitive and Specific: Demonstrated the highest true positive and lowest false positive rates compared to competing kits
• Easy-to-use: Includes ready-to-use primer mix and positive control
• 6X less expensive per sample compared to competing kits (Russel et al., 2020)
• Out-performs Sigma and Lonza's kits (Russel et al., 2020)
• Combine with our bestselling Mycoplasma Elimination Cocktail (Cat. No. G398) to eliminate 70+ species of Mycoplasma

Customer Testimonial

"The mycoplasma detection kit we received from ABM worked very well. It was fast, easy to use, and, with the included controls, the results were very easy to interpret. We will purchase this kit again the next time we are in need of mycoplasma detection."

- Haylie Romero, University of California San Diego

An official publication by a national public health agency finds our kit out-performs Sigma and Lonza's kits:

Russell, B. J., Horiuchi, K., Velez, J. O., Goodman, C. H., & Johnson, B. W. (2020). Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management. Journal of virological methods, 276, 113769. https://doi.org/10.1016/j.jviromet.2019.113769

Why test your cells for Mycoplasma?

Due to its small size, Mycoplasma can be present in your growth media without being visibly detected. This can cause significant errors in your experiments due to its effects on:

• proliferation
• metabolism
• gene synthesis & processing
• adhesion properties
• and more

Many publishers now request Mycoplasma testing to be reported along with manuscript submissions to ensure accuracy in data reporting. Visit our learning resources center to read more about Mycoplasma contamination.