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qPCR Retrovirus Titer Kit

Cat. No.
G949
Unit
100 rxn
Cat. No. G949
Name qPCR Retrovirus Titer Kit
Unit
100 rxn
Category PCR, qPCR, and RT
Description abm’s qPCR Retrovirus Titer Kit is a one-step assay which employs a quick RNA extraction step that is followed by RT-qPCR. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits on the market. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments. MasterMix contains dye comparable to SYBR Green™ and EvaGreen™.

Product Component Quantity
BlasTaq™ 2X qPCR Titer MasterMix 1.25 ml
Primer Mix 100 rxn (200 µl)
Standard Control DNA 50 µl
Virus Lysis Buffer 800 µl
ROX Reference Dye 15 µl
Nuclease-Free H2O 2 x 1.0 ml

 

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

 

Storage Condition Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.
Note qPCR Retrovirus Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

MACHINE TYPE MACHINE
High ROX Machines
  • ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series
  • Biometra TOptical
  • Fluidigm BioMark™
  • Wafergene SmartChip System
  • TianLong TL998 System
Low ROX Machines
  • ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7
  • BioGene InSyte™
  • Illumina Eco
  • Analytikjena qTower Series
  • Stratagene® Mx3000, Mx3005, Mx4000
No ROX machines
  • BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™
  • Roche LightCycler® (480, 1536, Nano)
  • MJ Research Opticon™, Opticon™ 2, Chromo® 4
  • Eppendorf™ Realplex 4
  • BioGene SynChron™
  • Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
  • Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)
  • Cepheid SmartCyler, GeneXpert
  • Enigma™ ML
  • Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS
  • Qiagen Rotor-Gene™ (Q, 6000)
  • Takara Dice™
  • Thermo Scientific PikoReal
  • DNA-Technology DT96, DTlite, DT-322
  • Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
  • Bioneer Exicycler™

One important thing to note is that the infection success is dependent on the cell type. Some cells may be more susceptible than others for viral infection. Thus, the same number of infectious units may perform differently on different cell types.

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