G236 OneScript® Plus cDNA Synthesis Kit

OneScript® Plus cDNA Synthesis Kit

Cat. No.	G236
Unit	100 rxn

Cat. No.

G236

Name

OneScript® Plus cDNA Synthesis Kit

Unit

100 rxn

Category

PCR, qPCR, and RT

Description

(Equivalent to Cat. No. G233, G234, G235, and G491.)OneScript^® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript^® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

Key Features

Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
Robust cDNA synthesis from any RNA template
High reproducibility and excellent yield
Employs abm's OneScript^® Plus Reverse Transcriptase (Cat. No. G237)
Earn 2X the rewards points

ISO 13485:2016 MDSAP Certified
Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Product Component	Quantity
OneScript^® Plus Reverse Transcriptase	100 rxn (100 µl)
5X RT Buffer	400 µl
Oligo(dT) (10 μM)	100 µl
Random Primers (10 μM)	100 µl
dNTP (10 mM)	100 µl
Nuclease-Free H₂O	2 x 1.0 ml

Note: This product is abm’s next generation of cDNA Synthesis Kits and is functionally equivalent to Cat. No. G233, G234, G235, and G491 (OneScript™ and OneScript™ Plus cDNA Synthesis Kits), with improved performance. Contact our customer service team technical@abmgood.com for more information.

Application

Synthesizing cDNA from ssRNA
DNA primer extension
Sequencing dsDNA
Constructing cDNA library
Producing template for use in RT-PCR
Labelling 3'-end of duplex DNA via end-filling reactions
Generating probes for hybridization

Storage Condition

Store at -20°C.

Datasheet

G236 OneScript® Plus cDNA Synthesis Kit Datasheet

Search CoA here

MSDS

G236 MSDS

Supporting Protocol

Other

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Menon, V et al. "The contribution of visceral fat to improved insulin signaling in Ames dwarf mice" Aging Cell. 13(3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription.
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ŞEN, A., Ayar, B., Yilmaz, A., ACAR, Ö. Ö., Turgut, G. C., & TOPÇU, G. "Natural diterpenoid alysine A isolated from Teucrium alyssifolium exerts antidiabetic effect via enhanced glucose uptake and suppressed glucose absorption" Turkish Journal of Chemistry 43(5):1350-1364 (2019).
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Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
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