| CAT.NO | UNIT |
|---|---|
| G891 | 5.0 ml ( 4 x 1.25 ml) |
| G892 | 5 x G891 ( 25 ml) |
Specifications
| Description | BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaqTM DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. | |
| BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors. | ||
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Key Features |
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| PRODUCT COMPONENT | QUANTITY | |
| BlasTaq™ 2X qPCR MasterMix |
500 rxn (4 x 1.25 ml) |
|
| ROX Reference Dye |
50 µl |
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| SKU | G891 | |
| Concentration | 2X | |
| Storage Condition | Store at -20 °C. | |
| Unit quantity | 500 rxn (4 x 1.25 ml) | |
| Note | BlasTaq™ 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below. | |
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MACHINE TYPE |
MACHINE | |
| High ROX Machines | ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series | |
| Biometra TOptical | ||
| Fluidigm BioMark™ | ||
| Wafergene SmartChip System | ||
| TianLong TL998 System | ||
| Low ROX Machines | ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7 | |
| BioGene InSyte™ | ||
| Illumina Eco | ||
| Analytikjena qTower Series | ||
| Stratagene® Mx3000, Mx3005, Mx4000 | ||
| No ROX machines | BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™ | |
| Roche LightCycler® (480, 1536, Nano) | ||
| MJ Research Opticon™, Opticon™ 2, Chromo® 4 | ||
| Eppendorf™ Realplex 4 | ||
| BioGene SynChron™ | ||
| Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600) | ||
| Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat) | ||
| Cepheid SmartCyler, GeneXpert | ||
| Enigma™ ML | ||
| Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS | ||
| Qiagen Rotor-Gene™ (Q, 6000) | ||
| Takara Dice™ | ||
| Thermo Scientific PikoReal | ||
| DNA-Technology DT96, DTlite, DT-322 | ||
| Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680) | ||
| Bioneer Exicycler™ | ||
qPCR and One-Step RT MasterMixes
Streamline your qPCR, and RT-PCR with abm’s MasterMixes. Our specially formulated MasterMixes contain our next generation enzymes as well as all the ingredients you need premixed into an optimized format, significantly simplifying your workflow. Enjoy reduced handling errors, high reproducibility and excellent yields.
Premixed and ready-to-use
Simple and streamlined for high-throughput use
Reduce handling errors
Outstanding Thermostability
BlasTaq™ Probe One-Step RT-qPCR has superb sensitivity and signal-to-noise ratio. 20 µl One-Step RT-qPCR reactions (20 ng, 2 ng, 200 pg, 20 pg, or 2 pg of RNA per reaction) were carried out with abm’s BlasTaq™ One-Step RT-qPCR (Cat. No. G598) for GAPDH amplification.
| BlasTaq™ One-Step RT-qPCR | G598 |
| BlasTaq™ Probe One-Step RT-qPCR | G596 |
| MegaFi™ One-Step RT-PCR* | G597 |
| * Contains OneScript™ Hot Reverse Transcriptase (Cat. No. G593) | |
Documents
Supporting Protocol
MSDS
Other
FAQs
- Is the dye set-up similar to SYBR Green™ & EvaGreen™?
Yes, it is comparable.
- What are the advantages to using BlasTaq™ 2X qPCR MasterMix?
It is a ready-to-use qPCR MasterMix in which only template and primers need to be added by the end user. BlasTaq™ has super speed performance to achieve results less than a hour. ROX Reference Dye is offered on the side to give more flexibility to the user for your qPCR set up.
References
