YO3-3PEG-Biotin Fluorophore

YO3-3PEG-Biotin Fluorophore

Cat. No.	G957
Unit	0.5 mg/ml (500 µl)

Cat. No.

G957

Name

YO3-3PEG-Biotin Fluorophore

Unit

0.5 mg/ml (500 µl)

Category

RNA Tracking (RNA Mango)

Description

YO3-3PEG-Biotin is a small bifunctional fluorophore that has low unbound fluorescence. When bound to Mango aptamers, it exhibits peak excitation maxima of 580 nm (with additional excitation at 260 nm) and peak fluorescence emission of 620 nm. Mango aptamers enhance the fluorescence of YO3-3PEG-Biotin (binding requires KCl, 61-fold brighter with Mango III A10U), emitting in the orange region of the visible spectrum (unpublished data from Unrau Laboratory). YO3-3PEG-Biotin may serve as a FRET acceptor, when paired with GFP-emitting fluorophores¹.

RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The TO dye has a number of other desirable properties including:

Small size
Lack of toxicity
Plasma and nuclear membrane permeability
Short intracellular half-life
The accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure

TO3-3PEG-Biotin has binding affinities to Mango I, Mango II, Mango III, and Mango IV aptamers.

The most advanced RNA tracking, visualization and pull down technology.Technology for studying the diverse cellular roles of RNA has lagged behind the tools for studying DNA and proteins, but innovative researchers are working to change that! One such researcher is Dr. Peter Unrau of Simon Fraser University. He and his team have created RNA Mango, a novel technology with a number of useful applications.

Storage Condition

Stored at -20°C and protect from light.

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