BlasTaq™ 2X qPCR MasterMix
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Cat. No.
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G891 |
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| Unit |
500 rxn (4 x 1.25 ml)
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| Cat. No. | G891 | ||||||||
| Name | BlasTaq™ 2X qPCR MasterMix | ||||||||
| Unit | 500 rxn (4 x 1.25 ml) | ||||||||
| Category | PCR, qPCR, and RT | ||||||||
| Description | Equivalent to Cat. No. MasterMix-5L, -5R, -5S, -EL, -ER, -ES, KL, -KR, -KS, -LR, -mL, -mR, -mS, -R, and -S) BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors. Product Features:
ISO 13485:2016 MDSAP Certified
Customer Testimonial "Very nice product. I always get consistent results. Even low expression genes can be quantified easily. Compatible with both standard and fast reaction protocols." - Dr. Vivek Vaish, Pune University
Note: This product is abm’s next generation of dye-based qPCR MasterMixes and is functionally equivalent to BrightGreen 5X qPCR MasterMixes (Cat. No. MasterMix-5L, MasterMix-5R, MasterMix-5S), BrightGreen Express 2X qPCR MasterMixes (Cat. No. MasterMix-EL, MasterMix-ER, MasterMix-ES), KiloGreen 2X qPCR MasterMixes (MasterMix-KL, MasterMix-KR, MasterMix-KS), BrightGreen 2X qPCR MasterMixes (Cat. No. MasterMix-LR, MasterMix-R, MasterMix-S) and BrightGreen miRNA qPCR MasterMixes (MasterMix-mL, MasterMix-mR, MasterMix-mS), with improved performance. Contact our customer service team technical@abmgood.com for more information.
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| Concentration | 2X | ||||||||
| Storage Condition | Store at -20 °C. This product is stable for 2 years from the date of shipping if stored and handled properly. | ||||||||
| Note | BlasTaq™ 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
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Is the dye set-up similar to SYBR Green™ & EvaGreen™?
Yes, it is comparable.
What are the advantages to using BlasTaq™ 2X qPCR MasterMix?
It is a ready-to-use qPCR MasterMix in which only template and primers need to be added by the end user. BlasTaq™ has super speed performance to achieve results less than a hour. ROX Reference Dye is offered on the side to give more flexibility to the user for your qPCR set up.
- Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. DOI:10.1101/2020.12.20.423533
- Yang, Y. W., Meng, X., Meng, Y. Y., Tang, H. K., Cheng, M. H., Zhang, Z. Q., Xu, W. Q., & Long, W. (2021). ceRNA regulatory network of FIH inhibitor as a radioprotector for gastrointestinal toxicity by activating the HIF-1 pathway. Molecular Therapy - Nucleic Acids, 25, 173–185. DOI: 10.1016/j.omtn.2021.05.008
- Luo, N., Cheng, W., Zhou, Y., Gu, B., Zhao, Z., & Zhao, Y. (2021). Screening Candidate Genes Regulating Placental Development from Trophoblast Transcriptome at Early Pregnancy in Dazu Black Goats (Capra hircus). Animals, 11(7), 2132. DOI: 10.3390/ani11072132
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