페이지 선택

PCR Sure™ Kit

Cat. No.
G065
Unit
8 x 10 reactions
Cat. No. G065
Name PCR Sure™ Kit
Unit 8 x 10 reactions
Category PCR, qPCR, and RT
Description It has been well established that many variables can affect a PCR including template structures, inhibitors, primer design, annealing temperature, and magnesium concentration. The PCR Sure™ Kit is developed to simplify the PCR optimization process. The system consists of multiple thermostable DNA polymerases pre-mixed with 8 optimized buffers in a 2X MasterMix format, saving a great amount of time in PCR set-up. To find the optimal conditions for your difficult PCR, all you need to do is mix templates, primers, and H2O with the PCR Sure™ 2X MasterMixes.

 

Product Component Quantity
PCR Sure™ 2X MasterMix 1 125 µl
PCR Sure™ 2X MasterMix 2 125 µl
PCR Sure™ 2X MasterMix 3 125 µl
PCR Sure™ 2X MasterMix 4 125 µl
PCR Sure™ 2X MasterMix 5 125 µl
PCR Sure™ 2X MasterMix 6 125 µl
PCR Sure™ 2X MasterMix 7 125 µl
PCR Sure™ 2X MasterMix 8 125 µl

 

Additional Materials Required

  • Template DNA
  • Primers
  • Nuclease-free H2O
Storage  Condition Store at -20°C.

The MgCl2 is already incorporated into the reaction mix so MgCl2 does not need to be added. Very rarely, an occasional customer might want to increase the MgC12 concentration, so we included it as a courtesy.

  • Chaturvedi, , NK. et al. "Experimental validation of predicted subcellular localizations of human proteins" BMC research notes 7 (1):912 (2014). PubMed: 25510246.

This product has no review yet.

 

Controls and Related Product:

SafeView™ Classic


Cat. No.
G108
Unit 1.0 ml

Safe-Green™


Cat. No.
G108-G
Unit 1.0 ml

Safe-Red™ Gel


Cat. No.
G680
Unit 1.0 ml

T4 DNA Ligase


Cat. No.
G467
Unit 200 U (200 μl)

Safe-Red™


Cat. No.
G108-R
Unit 1.0 ml

DNA Purification Magnetic Beads (5 ml)


Cat. No.
G950
Unit 4 x 1.25 ml

Column-Pure Gel and PCR Clean-Up Kit


Cat. No.
D516
Unit 100

Pro Ligation-Free Cloning Kit (20 reactions)


Cat. No.
E086
Unit 20 Reactions