Anti-V5 Tag Antibody (Goat Polyclonal)
CAT.NO | UNIT |
---|---|
G190 | 100 μg |
SKU | G190 |
---|---|
Unit quantity | 100 μg |
Clone | N/A |
Raised in | Goat |
Clonality | Tag |
Isotype | IgG |
Species Reactivity | Other |
Specificity | Anti-V5 Antibody detects (GKPIPNPLLGLDST) tag fusion protein. |
Immunogen | Goats were immunized with a synthetic peptide representing amino acid residues 95 to 108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. |
Applications | Western Blots: Colorimetric detection: 1:1,000 - 1:10,000 Chemiluminescent detection: 1:1,000 - 1:30,000. ELISA: Coating: 1:100 - 1:500 Primary: 1:1,000 - 1:30,000 Immunochemistry: 1:100 - 1:400 In some cases, the antibody may be diluted further than indicated. |
Recommended Dilutions | Western Blots: Colorimetric detection: 1:1,000 - 1:10,000 Chemiluminescent detection: 1:1,000 - 1:30,000. ELISA: Coating: 1:100 - 1:500 Primary: 1:1,000 - 1:30,000 Immunochemistry: 1:100 - 1:400 In some cases, the antibody may be diluted further than indicated. |
Formulation | Liquid |
Concentration | 100ug/100ul |
Storage Condition | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
Storage Buffer | PBS, pH 7.4 with 0.05% sodium azide. |
Guarantee | abm guarantees that all our Anti-V5 Tag Antibody (Goat Polyclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
Supporting Protocol
MSDS
QC
Other
- Gueller, S et al. "Adaptor protein Lnk associates with Tyr(568) in c-Kit" Biochem. J. 415:241-5 (2008). PubMed: 18588518. Application: Western Blot.