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Anti-V5 Tag Antibody (Goat Polyclonal)

 

CAT.NO UNIT
G190 100 μg 
SKU G190
Unit quantity 100 μg
Clone N/A
Raised in Goat
Clonality Tag
Isotype IgG
Species Reactivity Other
Specificity Anti-V5 Antibody detects (GKPIPNPLLGLDST) tag fusion protein.
Immunogen Goats were immunized with a synthetic peptide representing amino acid residues 95 to 108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Applications Western Blots: Colorimetric detection: 1:1,000 - 1:10,000 Chemiluminescent detection: 1:1,000 - 1:30,000. ELISA: Coating: 1:100 - 1:500 Primary: 1:1,000 - 1:30,000 Immunochemistry: 1:100 - 1:400 In some cases, the antibody may be diluted further than indicated.
Recommended Dilutions Western Blots: Colorimetric detection: 1:1,000 - 1:10,000 Chemiluminescent detection: 1:1,000 - 1:30,000. ELISA: Coating: 1:100 - 1:500 Primary: 1:1,000 - 1:30,000 Immunochemistry: 1:100 - 1:400 In some cases, the antibody may be diluted further than indicated.
Formulation Liquid
Concentration 100ug/100ul
Storage Condition This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer PBS, pH 7.4 with 0.05% sodium azide.
Guarantee abm guarantees that all our Anti-V5 Tag Antibody (Goat Polyclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.

Supporting Protocol
MSDS
QC
Other

  • Gueller, S et al. "Adaptor protein Lnk associates with Tyr(568) in c-Kit" Biochem. J. 415:241-5 (2008). PubMed: 18588518. Application: Western Blot.