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Anti-Myc Tag Antibody (Mouse Monoclonal)

 

 

CAT.NO UNIT
G019 100 μg 
Description The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]
SKU G019
Unit quantity 100 μg
Clone Myc.A7
Raised in Mouse
Clonality Tag
Isotype IgG1
Species Reactivity Other
Specificity Myc-tag Monoclonal Antibody. Recognises over-expressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Immunogen A synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH.
Applications Western Blot (1:1000), Immunoprecipitation and Immunostaining (1:200). Other dilutions will need optimization by end user.
Recommended Dilutions Western Blot (1:1000), Immunoprecipitation and Immunostaining (1:200). Other dilutions will need optimization by end user.
Formulation Liquid
Concentration 100ug/100ul
Storage Condition This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer PBS, pH 7.4 with 0.05% sodium azide.
Guarantee abm guarantees that all our Anti-Myc Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.

Supporting Protocol
MSDS
QC
Other

We use Protein A affinity beads for all tag antibody purification.

Cat#L001 - Myc Tag Cell Lysate is a great positive control for this antibody. This control contains 293 cell lysate that were transfected with a Myc-tagged minigene, sized 37 kDa.

Yes, this antibody can work on the stated application. End user will need to optimized the conditions for their own unique experiments.

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  • Shi, XH et al. "The expression and localization of N-myc downstream-regulated gene 1 in human trophoblasts" PLoS One 8 (9):e75473 (2013). DOI: 10.1371/journal.pone.0075473. Application: Western Blot.
  • Mo, Y.Y et al. "MicroRNA-145 Suppresses Cell Invasion and Metastasis by Directly Targeting Mucin 1" Journal of Cancer Res 70 (1):278-287 (2010). DOI: 10.1158/0008-5472. Application: Western Blot.
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  • Shi, XH et al. "The expression and localization of N-myc downstream-regulated gene 1 in human trophoblasts" PLoS ONE 8:e75473 (2013). PubMed: 24066183.
  • Zhang, A et al. "The human long non-coding RNA-RoR is a p53 repressor in response to DNA damage" Cell Res. 23(3):340-50 (2013). DOI: 10.1038/cr.2012.164. PubMed: 23208419.
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  • Min, H. J., Cui, L. H., Oh, T. R., Kim, J. H., Kim, T. W., & Kim, W. T. "Os BZR 1 turnover mediated by Os SK 22‐regulated U‐box E3 ligase Os PUB 24 in rice BR response" The Plant Journal 99(3):426-438 (2019).
  • Singh, R., Dangol, S., Chen, Y., Choi, J., Cho, Y. S., Lee, J. E., ... & Jwa, N. S. "Magnaporthe oryzae effector AVR-Pii helps to establish compatibility by inhibition of the rice NADP-malic enzyme resulting in disruption of oxidative burst and host innate immunity"  Molecules and cells  39(5):426 (2016).