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abm Antibodies Tag Antibodies Anti-His Tag Antibody (Rabbit Polyclonal)
Anti-His Tag Antibody (Rabbit Polyclonal)
| CAT.NO | UNIT |
|---|---|
| G162 | 100 μg / 1.0 mg |
| SKU | G162 |
|---|---|
| Unit quantity | 100 μg / 1.0 mg |
| Clone | Polyclonal |
| Raised in | Rabbit |
| Clonality | Tag |
| Isotype | IgG |
| Species Reactivity | Other |
| Specificity | His-Tag polyclonal antibody detects His-tagged proteins overexpressed in cells. The antibody recognizes the His-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells. |
| Immunogen | The antiserum was produced by immunizing rabbit with synthesized peptide containing the His epitope (H-H-H-H-H-H). |
| Applications | ELISA 1: 2500; WB 1:500-1000; IP 5 μg/sample; IF 5μg/mL; IHC 5 μg/mL |
| Recommended Dilutions | ELISA 1: 2500; WB 1:500-1000; IP 5 μg/sample; IF 5μg/mL; IHC 5 μg/mL |
| Formulation | Liquid |
| Concentration | 100ug/100ul |
| Storage Condition | This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Storage Buffer | PBS, pH 7.4 with 0.02% Sodium Azide |
| Guarantee | abm guarantees that all our Anti-His Tag Antibody (Rabbit Polyclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Purity | Affinity purified |
Supporting Protocol
MSDS
QC
Other
- Zhou, L et al. "Microenvironmental regulation of BRCA1 gene expression by c-Jun and Fra2 in premalignant human ovarian surface epithelial cells" Mol. Cancer Res. 11:272-81 (2013). PubMed: 23339184. Application: Western Blot, Immunofluorescence.
