Anti-Beta-Tubulin Monoclonal Loading Control Antibody

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CAT.NO	UNIT
G098	100 μg

SKU	G098
Unit quantity	100 μg
Clone	H3
Raised in	Mouse
Clonality	Monoclonal
Isotype	IgG
Species Reactivity	Human
Specificity	Recognize β-tubulin from Human, Mouse, Xenopus, Drosophila, Kangaroo rat, and Chylamydomonas.
Immunogen	Recombinant β-tubulin protein from bacteria.
Applications	Western blot at dilution of 1:1000 Immunofluorescence staining at 1:100 dilution. Immunohistochemistry staining at 1:6000 dilution. It is very useful as a loading control for quantitive western blotting.
Recommended Dilutions	Western blot at dilution of 1:1000 Immunofluorescence staining at 1:100 dilution. Immunohistochemistry staining at 1:6000 dilution. It is very useful as a loading control for quantitive western blotting.
Formulation	Liquid
Concentration	100ug/100ul
Storage Condition	This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Guarantee	abm guarantees that all our Anti-Beta-Tubulin Monoclonal Loading Control Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.

Supporting Protocol
MSDS
QC
Other

The antigen is full protein and the epitope sequence is undetermined.

Liu, PQ et al. "Generation of a triple-gene knockout mammalian cell line using engineered zinc-finger nucleases" Biotechnol Bioeng 106 (1):97-105 (2010). PubMed: 20047187. Application: Western Blot.
Kwon, YC et al. "The ribonuclease l-dependent antiviral roles of human 2′,5′-oligoadenylate synthetase family members against hepatitis C virus" FEBS Lett. 587 (2):156-164 (2013). DOI: 10.1016/j.febslet.2012.11.010. Application: Immunoblot.
Jung, ES et al. "Astrocyte-Originated ATP Protects Aβ1-42-Induced Impairment of Synaptic Plasticity" J Neurosci 32 (9):3081-3007 (2012). DOI: 10.1523/JNEUROSCI.6357-11.2012. Application: Immunoblot.
Liu, Y et al. "Ubiquilin-1 protects cells from oxidative stress and ischemic stroke caused tissue injury in mice" J Neurosci 34:2813-21 (2014). PubMed: 24553923.
Cornell, R. B., Taneva, S. G., Dennis, M. K., Tse, R., Dhillon, R. K., & Lee, J. "Disease-linked mutations in the phosphatidylcholine regulatory enzyme CCTα impair enzymatic activity and fold stability" Journal of Biological Chemistry 294(5):1490–1501 (2018). DOI: 10.1074/jbc.ra118.006457.