CRISPR is the most versatile technology for genome editing and abm offers the largest selection of Cas9 proteins for CRISPR experiments. Cas9 RNPs (Cas9 proteins in complex with in vitro transcribed sgRNA) can be directly tranfected into cells to achieve gene editing.

This method has several advantages over other Cas9 delivery methods:

• There is no transcription or translation required so editing is much more rapid and transient
• Cas9 proteins are compatible with a wide range of organisms without consideration such as codon optimization or promoter compatibility
• No plasmid or virus means no risk of Cas9 or sgRNA integration into the genome

Cas9 proteins and sgRNA can also be used for cutting DNA in vitro. Applications for this technique include pre-screening of sgRNA candidates on PCR amplified versions of the target gene of interest, or digestion of plasmids where no restriction site is available.

CRISPR Experimental Design Tool
Experimental recommendations tailored to your model system.