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Cas9 Proteins

CRISPR is the most versatile technology for genome editing and abm offers the largest selection of Cas9 proteins for CRISPR experiments. Cas9 RNPs (Cas9 proteins in complex with in vitro transcribed sgRNA) can be directly tranfected into cells to achieve gene editing.

This method has several advantages over other Cas9 delivery methods:

  • There is no transcription or translation required so editing is much more rapid and transient
  • Cas9 proteins are compatible with a wide range of organisms without consideration such as codon optimization or promoter compatibility
  • No plasmid or virus means no risk of Cas9 or sgRNA integration into the genome

Cas9 proteins and sgRNA can also be used for cutting DNA in vitro. Applications for this technique include pre-screening of sgRNA candidates on PCR amplified versions of the target gene of interest, or digestion of plasmids where no restriction site is available.

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Sku: K089
Unit: 6.5µg (50pmol/ 50µL)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the…

 


Sku: K189
Unit: 32.5µg (250pmol/ 50µL)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the…

 


Sku: K188
Unit: 380 μg (2.5 nmol/ 250µL)
Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases. Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9. Cpf1 can be used with a…

 


Sku: K187
Unit: 380 μg (2.5 nmol/ 250µL)
Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases.Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9. Cpf1 can be used with a…

 


Sku: K186
Unit: 47µg (250pmol/ 25µL)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 Null Mutant Protein is…

 


Sku: K149
Unit: 47µg (250pmol/ 25µL)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR…

 


Sku: K148
Unit: 47µg (250pmol/ 25µL)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind…

 


Sku: K147
Unit: 32.5µg (250pmol/ 25µL)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The saCas9 Null Mutant Protein is…

 


Sku: K146
Unit: 32.5µg (250pmol/ 25µL)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The saCas9 Null Mutant Protein is…

 


Sku: K144
Unit: 32.5µg (250pmol/ 25µL)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the…

 

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