| CAT.NO | UNIT |
|---|---|
| G595 | 500 rxn / 20ul |
Specifications
| Description | BlasTaqTM HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. | |
| During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaqTM has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaqTM can be used with TA cloning vectors. | ||
| PRODUCT COMPONENT | QUANTITY | |
| BlasTaqTM HotStart DNA Polymerase | 400 rxn (200 µl) | |
| 5X BlasTaqTM Buffer 1 | 2 x 1.0 ml | |
| 1 Buffer contains 1.5 mM Mg2+. | ||
| SKU | G595 | |
| Storage Condition | Store at -20°C. | |
| Unit quantity | 400 rxn | |
Eliminate non-specific amplification and primer-dimer formation with our next generation BlasTaq™ HotStart DNA Polymerase. This formulation includes a proprietary antibody that blocks polymerase activity at low temperatures and dissociates at the denaturation step to restore enzyme activity.
No non-specific amplification or primer dimers
Improves yield of desired product
Simplifies reaction setup
Eliminates Non-specific Amplification
Super Fast Extension Speeds
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