Pro Ligation-Free Cloning Kit (20 reactions
|
Cat. No.
|
E026 |
|---|---|
| Unit | 2000 U (100 μl) |
| Cat. No. | E026 |
| Name | DpnI |
| Unit |
2000 U (100 μl)
|
| Category | Molecular Biology Enzymes and Kits |
| Description | The DpnI restriction enzyme digests DNA at GmeA↓TC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam+ E. coli strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60X more slowly than fully methylated dam sites. |
| Reaction Definition | 1X Universal Reaction Enzyme Reaction buffer (Cat. E204) and incubate at 37°C. |
| Note | Reaction Buffer Compatibility
Buffer 1: 100%, Buffer 2: 100%, Buffer 3: 75%, Universal Buffer: 100% Heat inactivation 65°C for 20 minutes. |
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