Anti-V5 Tag Antibody (Mouse Monoclonal)

HOME abm Antibodies Tag Antibodies Anti-V5 Tag Antibody (Mouse Monoclonal)

CAT.NO	UNIT
G189	100 μg

SKU	G189
Unit quantity	100 μg
Clone	V5.E10
Raised in	Mouse
Clonality	Tag
Isotype	IgG1
Species Reactivity	Other
Specificity	Anti-V5 Antibody detects (GKPIPNPLLGLDST) tag fusion protein.
Immunogen	Anti-V5 is a mouse monoclonal antibody, affinity purified from culture supernatant of hybridoma cells grown in a bioreactor.
Applications	Western blot analysis: 1:1000 ELISA: 1:5000-10,000 IHC: 1:50-1:100. Other applications will need optimization from end users.
Recommended Dilutions	Western blot analysis: 1:1000 ELISA: 1:5000-10,000 IHC: 1:50-1:100. Other applications will need optimization from end users.
Formulation	Liquid
Concentration	100ug/100ul
Storage Condition	This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Guarantee	abm guarantees that all our Anti-V5 Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Purity	Protein A affinity chromatography

Supporting Protocol
MSDS
QC
Other

We use Protein A affinity beads for all tag antibody purification.

Gueller, S et al. "Adaptor protein Lnk associates with Tyr568 in c-Kit" Biochem J 413 (2):241-245 (2008). DOI: 10.1042/BJ20080102. Application: Western Blot.
Gueller, S et al. "Adaptor protein Lnk binds to PDGF receptor and inhibits PDGF-dependent signaling" Exp Hematol. 39 (5):591-600 (2011). DOI: 10.1016/j.exphem.2011.02.00. Application: Western Blot.
Gueller, S et al. "Adaptor protein Lnk inhibits c-Fms-mediated macrophage function" J Leukoc Biol 88 (4):699-706 (2010). DOI: 0.1189/jlb.0309185. Application: Western Blot.
Gery , S et al. "Expression of the adaptor protein Lnk in leukemia cells" Exp Hematol. 37(5):585-592.e2 (2009). DOI: 10.1016/j.exphem.2009.01.009. PubMed: 19375649. Application: Western Blot.
Al-Maqtari, AT et al. "Promoting differentiation and survival of human ckit+cardiac progenitor cells ex vivo." Phd Dissertation (University of Louisville) : (2015). DOI: http://ir.library.louisville.edu/etd/2228/.