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Anti-GFP Tag Antibody (Goat Polyclonal)

 

CAT.NO UNIT
G095 100 μg
SKU G095
Unit quantity 100 μg
Clone N/A
Raised in Goat
Clonality Tag
Isotype IgG
Species Reactivity Other
Specificity Recognize GFP and its variants.
Immunogen The anti-GFP immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Applications ELISA 1:40,000; WESTERN BLOT 1:400 - 1:2,000; IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000; IF MICROSCOPY 1:1,000; IP 1:200
Recommended Dilutions ELISA 1:40,000; WESTERN BLOT 1:400 - 1:2,000; IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000; IF MICROSCOPY 1:1,000; IP 1:200
Formulation Liquid
Concentration 100ug/100ul
Storage Condition This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer PBS, pH 7.4 with 0.05% sodium azide.
Guarantee abm guarantees that all our Anti-GFP Tag Antibody (Goat Polyclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.

Supporting Protocol
MSDS
QC
Other

  • Sutton, LM et al. "Hip14l-deficient mice develop neuropathological and behavioural features of Huntington disease" Hum Mol Genet 22 (3):452-465 (2013). DOI: 10.1093/hmg/dds441. Application: Immunoprecipitation.
  • Luchniak, A et al. "Structure-function analysis of yeast" Methods Cell Biol 115:355-374 (2013). DOI: 10.1016/B978-0-12-407757-7.00022-0. Application: SDS-PAGE.