abm‘s CRISPR Knockout sgRNA AAV Viruses have a broad host range and low immunogenicity, perfect forin vivoCRISPR experiments. AAV is non-integrating, so there is less risk of interfering with host gene expression. We offer a collection of Knockout AAVs that express sgRNAs targeting any human, mouse, or rat gene, compatible with either spCas9 or saCas9. In addition to sgRNA-only vectors, we also offer an All-In-One AAV option, which expresses sgRNA and saCas9 nuclease from the same vector.

Key Features

• Collection of sgRNAs for knockout of your gene of interest with either saCas9 or spCas9. Human, mouse, and rat gene targets are available.
• AAV delivery for low immunogenicity and non-integrating expression of sgRNA and/or saCas9.
• Available expressing sgRNA alone, or as an All-In-One construct which expresses both sgRNA and saCas9.
• Comes as a set or pool of three sgRNA AAVs for assurance of efficient gene knockout.
• Available as AAV serotypes 1 to 11 for high transduction efficiency in specific tissues.

For multiplex gRNA expression from lentiviral vector, use our Custom Multiplex gRNA Service.
For knockout of up to 100 target genes, take advantage of our pooled Custom Targeted Lentiviral sgRNA Library Service.

Performance/Data
Click to see our CRISPR Knockout Case Study, including experimental design and supporting data.