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Cas9-Expressing Cell Lines

CRISPR is the most versatile technology for genome editing. As such, abm offers a wide variety of Cas9 expressing stable cell lines. These cell lines only require the introduction of sgRNA: from our human, mouse or rat Genome-Wide sgRNA Libraries; from a custom sgRNA vector or virus; or even through transfection of in vitro transcribed sgRNA. CRISPR Knock in also requires the introduction of a repair template. Can’t find what you are looking for? Contact us for more info.

Features of abm’s Cas9 Expressing Cell Lines:

  • Available in popular cell line research models.
  • Cas9 expression pre-validated by qPCR and/or Western blot.
  • Simplified workflow: only sgRNA is needed.

Browse Products


Sku: T3453
Unit: 1×106 cells / 1.0 ml
To generate the Cas9 Expressing Immortalized Chicken Embryonic Fibroblast (DF1) Cell Line, Cas9-2A-PuroR was cloned into pT2KCAGGS using Gibson Assembly (NEB). The DF1 cells were then transfected, and selected for using puromycin. The constitutive…

 


Sku: T3454
Unit: 1×106 cells / 1.0 ml
Immortalized Human Cardiomyocytes – SV40 stably expressing Cas9

 


Sku: T3279
Unit: 1×106 cells / 1.0 ml
PC3-M cells stably expressing Cas9

 


Sku: T3275
Unit: 1×106 cells / 1.0 ml
NIH3T3 cells stably expressing Cas9

 


Sku: T3274
Unit: 1×106 cells / 1.0 ml
THP-1 cells stably expressing Cas9

 


Sku: T3273
Unit: 1×106 cells / 1.0 ml
Jurkat E6.1 cells stably expressing Cas9

 


Sku: T3272
Unit: 1×106 cells / 1.0 ml
L3.6pl cells stably expressing Cas9

 


Sku: T3452
Unit: 1×106 cells / 1.0 ml
Immortalized human microglia stably expressing Cas9

 


Sku: T3451
Unit: 1×106 cells / 1.0 ml
Immortalized human microglia stably expressing Cas9

 


Sku: T3450
Unit: 1×106 cells / 1.0 ml
Immortalized human skeletal muscle cells stably expressing Cas9
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