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2nd Gen. Packaging Mix & Lentifectin Combo Pack

CAT.NO UNIT  
LV003-G074  kit
 

Specifications

SKU LV003-G074
Unit quantity Kit
Description For the production of lentiviral particles, three components are generally required: 1) a lentiviral vector containing your inserts of interest (cDNA, shRNA, or miRNA), 2) one or two packaging vectors which contain all necessary viral structure proteins, 3) an envelope vector expressing Vesicular Stomatitis Virus (VSV) glycoprotein (G). In general, lentiviral vectors with a wildtype 5′ LTR need the 2nd generation packaging system because these vectors require TAT for activation. However, the 2nd generation packaging mix will also support the production of 3rd lentiviral expression vector with a chimeric 5′ LTR. All the lentiviral expression vectors marketed by ABM are 3rd generation with a chimeric of RSV promoter upstream of 5′-LTR.
Caution Not for diagnostic use.
Note NOT FOR RESALE without prior written consent of ABM. This product is distributed for laboratory research only.
Vector Map pLenti-P2ApLenti-P2B
Storage Condition Store G074 at 4°C upon arrival, do not freeze.  Store LV003 at -20°C upon arrival.

FAQs

References

  • Von Dwingelo, J., Chung, I. Y. W., Price, C. T., Li, L., Jones, S., Cygler, M., & Abu Kwaik, Y. “Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex” mBio 10(4): (2019). DOI: 10.1128/mbio.01942-19.