Porducts
|
Can be used to synthesize membrane proteins Membrane protein expression Kit
ProteoLiposome BD Expression Kit |
ProteoLiposome BD Expression Kit
Comes as a set with reagents necessary for membrane protein synthesis.
Perfectly suited to large-scale production of membrane protein.
Features:
- Membran protein has been synthesized using asolection liposome
-
Can be used for 6 reactions on bilayer-dialysis method 2.5 ml reaction scale.
- Target proteins can be confirmed through SDS-PAGE/CBB staining
Recommended for:
-
Users who require yields of membrane protein several miligrams.
-
Users who want to perform antigen production using membrane protein antibodies.
-
Users who want to perform interaction analysis.
-
User who want to shorten the time taken by membrane protein synthesis.
*This kit does not come with a dialysis device included.
A separate Slide-A-LyzerTM MINI Dialysis Devices, 10K MWCO (Thermo Fisher Scientific Inc.) is required.
Flow of operations
Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.
Specification
| Product Number | Product Name | Size | Instruction manual | Safety Data Sheet | |
|---|---|---|---|---|---|
| CFS-TRI-PLE-BD | ProteoLiposome BD Expression Kit | 1 set |  |
|
Product Composition
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
WEPRO7240 |
240 OD |
1 mL |
1 |
| SUB-AMIX SGC S1* | 40x |
12.5 mL |
1 |
|
SUB-AMIX SGC S2* |
40x |
12.5 mL |
1 |
| SUB-AMIX SGC S3* | 40x |
12.5 mL |
1 |
| SUB-AMIX SGC S4* | 40x |
12.5 mL |
1 |
|
Creatine Kinase |
20 mg/mL |
20 µL |
1 |
|
NTPmix |
25 mM |
200 µL |
1 |
|
5×Transcription Buffer LM |
5x |
400 µL |
1 |
|
RNase Inhibitor |
80K unit/mL |
30 µL |
1 |
|
SP6 RNA Polymerase |
80K unit/mL |
30 µL |
1 |
| Asolectin Liposome, lyophilized |
－ |
10 mg |
6 |
Storage Condition : -80 ℃
* SUB-AMIX SGC S1-S4 each vary in their composition.
When producing a 1x concentration, adjust by adding the same quantity of each
