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WEPRO8240H (13C, 15N) Core Kit

Porducts

Can be used to synthesize stable isotope 13C and 15N labeled proteins

His-tagged Protein Recommendation

Protein Expression Kit

 

WEPRO8240H (13C, 15N) Core Kit


WEPRO8240H (13C, 15N) Core Kit

Comes as a set with reagents necessary for stable isotope labeled protein synthesis.

The translation buffer contains stable isotope 13C and 15N labeled amino acids.

There is no need for customers to prepare an amino acid solution.

Improved removal performance against His-like proteins composed of wheat germ.

Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO8240 (13C, 15N) Core Kit.

 

Features:

 

  • Synthesizes stable isotopes 13C and 15N labeled protein with over 99% efficiency.

  • There is no need for customers to prepare an amino acid solution.
  • Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.

  • Target proteins can be confirmed through SDS-PAGE/CBB staining.

 

 

Recommended for:

 

  • Users who require yields of stable isotopes 13C and 15N labeled protein ranging from a few hundred micrograms to several miligrams.

  • Users who want to perform structure analysis using Nuclear Magnetic Resonance (NMR).

 

Flow of operations

WEPRO7240 Expression Kit img en

Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.

Specification

Product Number Product Name Size   Instruction manual Safety Data Sheet
CFS-C8HCN WEPRO8240H (13C, 15N) Core Kit   1 set      

 

Product Composition

 

Reagent Name

Concentration

Volume

Quantity

WEPRO8240H

240 OD

1 mL

1

SUB-AMIX SGC (13C, 15N)  S1* 40x

600 µL

1
SUB-AMIX SGC (13C, 15N)  S2* 40x 600 µL 1
SUB-AMIX SGC (13C, 15N)  S3* 40x 600 µL 1
SUB-AMIX SGC (13C, 15N)  S4* 40x 600 µL 1

Creatine Kinase

20 mg/mL

20 µL

1
NTPmix

25 mM

120 µL

1

5×Transcription Buffer LM

5x

240 µL

1

RNase Inhibitor

80K unit/mL

15 µL

1

SP6 RNA Polymerase

80K unit/mL

15 µL 1
 
Storage Condition ;  -80 ℃

 

*SUB-AMIX SGC(13C, 15N)S1-S4 each vary in their composition.

 When producing a 1x concentration, adjust by adding the same quantity of each.