Porducts
![](http://itsbio.co.kr/wp-content/uploads/2020/08/WEPRO8240H_13C15N__Core_kit_2.png)
Can be used to synthesize stable isotope 13C and 15N labeled proteins
His-tagged Protein Recommendation
Protein Expression Kit
WEPRO8240H (13C, 15N) Core Kit
WEPRO8240H (13C, 15N) Core Kit
Comes as a set with reagents necessary for stable isotope labeled protein synthesis.
The translation buffer contains stable isotope 13C and 15N labeled amino acids.
There is no need for customers to prepare an amino acid solution.
Improved removal performance against His-like proteins composed of wheat germ.
Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO8240 (13C, 15N) Core Kit.
Features:
-
Synthesizes stable isotopes 13C and 15N labeled protein with over 99% efficiency.
- There is no need for customers to prepare an amino acid solution.
-
Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.
-
Target proteins can be confirmed through SDS-PAGE/CBB staining.
Recommended for:
-
Users who require yields of stable isotopes 13C and 15N labeled protein ranging from a few hundred micrograms to several miligrams.
-
Users who want to perform structure analysis using Nuclear Magnetic Resonance (NMR).
Flow of operations
Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.
Specification
Product Number | Product Name | Size | Instruction manual | Safety Data Sheet | |
---|---|---|---|---|---|
CFS-C8HCN | WEPRO8240H (13C, 15N) Core Kit | 1 set |
Product Composition
Reagent Name |
Concentration |
Volume |
Quantity |
---|---|---|---|
WEPRO8240H |
240 OD |
1 mL |
1 |
SUB-AMIX SGC (13C, 15N) S1* | 40x |
600 µL |
1 |
SUB-AMIX SGC (13C, 15N) S2* | 40x | 600 µL | 1 |
SUB-AMIX SGC (13C, 15N) S3* | 40x | 600 µL | 1 |
SUB-AMIX SGC (13C, 15N) S4* | 40x | 600 µL | 1 |
Creatine Kinase |
20 mg/mL |
20 µL |
1 |
NTPmix |
25 mM |
120 µL |
1 |
5×Transcription Buffer LM |
5x |
240 µL |
1 |
RNase Inhibitor |
80K unit/mL |
15 µL |
1 |
SP6 RNA Polymerase |
80K unit/mL |
15 µL | 1 |
Storage Condition ; -80 ℃
*SUB-AMIX SGC(13C, 15N)S1-S4 each vary in their composition.
When producing a 1x concentration, adjust by adding the same quantity of each.