WEPRO8240H (15N) Core Kit

Porducts

Can be used to synthesize stable isotope¹⁵N labeled proteins

His-tagged Protein Recommendation

Protein Expression Kit

WEPRO8240H (15N) Core Kit

Comes as a set with reagents necessary for stable isotope labeled protein synthesis.

The translation buffer contains stable isotope ¹⁵N labeled amino acids.

There is no need for customers to prepare an amino acid solution.

Improved removal performance against His-like proteins composed of wheat germ.

Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO8240 (¹⁵N) Core Kit.

Synthesizes as stable isotope ¹⁵N labeled protein with over 99% efficiency.
There is no need for customers to prepare an amino acid solution.
Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.
Target proteins can be confirmed through SDS-PAGE/CBB staining.

Users who require yields of stable isotopes ¹⁵N labeled protein ranging from a few hundred micrograms to several miligrams.
Users who want to perform analysis of Heteronuclear single-quantum correlation spectroscopy (HSQC) spectrum.

WEPRO7240 Expression Kit img en

Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.

Specification

Product Number	Product Name	Size		Instruction manual	Safety Data Sheet
CFS-C8HN	WEPRO8240H (¹⁵N) Core Kit	1 set

Reagent Name	Concentration	Volume	Quantity
WEPRO8240H	240 OD	1 mL	1
SUB-AMIX SGC (¹⁵N) S1*	40x	600 µL	1
SUB-AMIX SGC (¹⁵N) S2*	40x	600 µL	1
SUB-AMIX SGC (¹⁵N) S3*	40x	600 µL	1
SUB-AMIX SGC (¹⁵N) S4*	40x	600 µL	1
Creatine Kinase	20 mg/mL	20 µL	1
NTPmix	25 mM	120 µL	1
5×Transcription Buffer LM	5x	240 µL	1
RNase Inhibitor	80K unit/mL	15 µL	1
SP6 RNA Polymerase	80K unit/mL	15 µL	1

*SUB-AMIX SGC（¹⁵N）S1-S4 each vary in their composition.

When producing a 1x concentration, adjust by adding the same quantity of each.