Porducts
Can be used to synthesize 13C and 15N stable isotope-labeled proteins
Premium PLUS Expression Kit for MS
Recommended for first-time users and to confirm the expression of target proteins
Premium PLUS Expression Kit for MS
This starter kit has been designed to be user-friendly even for first-time users.
Our wheat germ cell-free expression system allows you to try stable isotope-labeled proteins easily.
Features:
- Synthesizes stable isotopes 13C and 15N as labeled lysine and arginine residues with over 99% efficiency.(Other amino acids are in unlabeled form).
- No need to purchase 13C and 15N stable isotope labeled amino acids separately.
- No need to prepare reagents
- Reagents necessary for each reaction are already pre-packaged in tubes
- Synthesize proteins with just a few pipetting steps
- Target proteins can be confirmed through SDS-PAGE/CBB staining
- Contains enough for 16 reactions on a 226 µl reaction scale
Recommended for:
- Users who want to confirm the expression of target stable isotope -labeled proteins easily using wheat germ cell-free expression system.
- Users who require internally standardized stable isotope labeled proteins for use in SRM/MRM analysis of fixed quantities of proteins.
The reagents necessary for each reaction are pre-packaged into tubes, so there is no need to prepare any reagents.
Simply cut off the number of tubes per reaction you need, and you are ready to go.
Flow of operations
Specification
| Product Number | Product Name | Size | Instruction manual | Safety Data Sheet | |
|---|---|---|---|---|---|
| CFS-PLUS-MS | Premium PLUS Expression Kit for MS | 1 set |  |
|
Product Composition
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
pEU-E01-MCS |
1 µg/µL |
5 µL |
1 |
| pEU-E01-DHFR（Positive Control） | 1 µg/µL | 5 µL | 1 |
| PCR primer SPU | 1 µM | 100 µL | 1 |
| PCR primer deSP6E01 | 10 nM | 100 µL | 1 |
| WEPRO9240 for MS*1 | – | 10 µL | 16 |
| SUB-AMIX SGC for MS*2 | 1x | 206 µL | 16 |
| Transcription Premix LM *3 | 1x | 18 µL | 16 |
| Aluminum seal | – | – | 2 |
Storage Condition : -80 ℃
*1 : WEPRO9240 for MS is based on the WEPRO8240 with the addition of 13C and 15N-labeled lysine and arginine, and Creatine Kinase.
*2 : SUB-AMIX SGC for MS includes 18 types of non-labeled amino acids excluding lysine and arginine.
*3 : Transcription Premix LM is based on Transcription Buffer LM, with the addition of NTP Mix, SP6 RNA Polymerase and RNase Inhibitor.
