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Premium PLUS Expression Kit for MS

Porducts

Can be used to synthesize 13C and 15N stable isotope-labeled proteins

 

Premium PLUS Expression Kit for MS


Recommended for first-time users and to confirm the expression of target proteins

Premium PLUS Expression Kit for MS

This starter kit has been designed to be user-friendly even for first-time users.

Our wheat germ cell-free expression system allows you to try stable isotope-labeled proteins easily.

 

 

Features:

 

  • Synthesizes stable isotopes 13C and 15N as labeled lysine and arginine residues with over 99% efficiency.(Other amino acids are in unlabeled form).
  • No need to purchase 13C and 15N stable isotope labeled amino acids separately.
  • No need to prepare reagents
  • Reagents necessary for each reaction are already pre-packaged in tubes
  • Synthesize proteins with just a few pipetting steps
  • Target proteins can be confirmed through SDS-PAGE/CBB staining
  • Contains enough for 16 reactions on a 226 µl reaction scale

 

 

Recommended for:

 

  • Users who want to confirm the expression of target stable isotope -labeled proteins easily using wheat germ cell-free expression system.
  • Users who require internally standardized stable isotope labeled proteins for use in SRM/MRM analysis of fixed quantities of proteins.

 

 

The reagents necessary for each reaction are pre-packaged into tubes, so there is no need to prepare any reagents.

Simply cut off the number of tubes per reaction you need, and you are ready to go.

 

 

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Flow of operations

 

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Specification

Product Number Product Name Size   Instruction manual Safety Data Sheet
CFS-PLUS-MS Premium PLUS Expression Kit for MS   1 set   btn download btn download

 

 

Product Composition

 

Reagent Name

Concentration

Volume

Quantity

pEU-E01-MCS

1 µg/µL

5 µL

1

pEU-E01-DHFR(Positive Control) 1 µg/µL 5 µL 1
PCR primer SPU 1 µM 100 µL 1
PCR primer deSP6E01 10 nM 100 µL 1
WEPRO9240 for MS*1 10 µL 16
SUB-AMIX SGC for MS*2 1x 206 µL 16
Transcription Premix LM *3 1x 18 µL 16
Aluminum seal 2
 
Storage Condition :  -80 ℃

 

*1 : WEPRO9240 for MS is based on the WEPRO8240 with the addition of 13C  and 15N-labeled lysine and arginine, and Creatine Kinase.

*2 : SUB-AMIX SGC for MS includes 18 types of non-labeled amino acids excluding lysine and arginine.

*3 : Transcription Premix LM is based on Transcription Buffer LM, with the addition of NTP Mix, SP6 RNA Polymerase and RNase Inhibitor.