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Porducts

Can be used to synthesize stable isotope labeled proteins

His-tagged Protein Recommendation

Protein Expression Kit

 

WEPRO8240H Core Kit


WEPRO8240H Core Kit

Comes as a set with reagents necessary for stable isotope labeled proteins synthesis.

Synthesises by adjusting stable isotope labeled proteins, according to the customer’s goals.

 

Improved removal performance against His-like proteins composed of wheat germ.

Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO8240 Core Kit.

 

* This kit does not come with isotope labeled amino acids included.

If the customer wants to prepare and adjust amino acids themselves, our order-made translation buffer can be used, requiring no separate amino acid adjustment. 

 

Features:

 

  • Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.

  • Target proteins can be confirmed through SDS-PAGE/CBB staining.

 

 

Recommended for:

 

  • Users who require yields of soluble protein ranging from a few hundred micrograms to several miligrams.

  • Users who want to progress with the synthesis of dozens of samples as soluble protein simultaneously.

  • Users who want to perform screening.

  • Users who want to perform structure analysis.

  • Users who want to perform interaction analysis.

  • Users who want to perform function analysis.

 

 

Flow of operations

 

WEPRO7240 Expression Kit img en

Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.

 

Specification

Product Number Product Name Size Instruction manual Safety Data Sheet
CFS-C8H WEPRO8240H Core Kit   1 set btn download

 

 

Product Composition

 

Reagent Name

Concentration

Volume

Quantity

WEPRO8240H

240 OD

1 mL

1

SUB-AMIX SGC NA S1* 40x

600 µL

1

SUB-AMIX SGC NA S2*

40x

600 µL

1

SUB-AMIX SGC NA S3*

40x

600 µL

1
SUB-AMIX SGC NA S4* 40x

600 µL

1

Creatine Kinase

20 mg/mL

20 µL

1

NTPmix

25 mM

120 µL

1

5×Transcription Buffer LM

5x

240 µL

1

RNase Inhibitor

80K unit/mL

15 µL

1

SP6 RNA Polymerase

80K unit/mL

15 µL

1
 
Storage Condition :  -80 ℃

 

*SUB-AMIX SGC NA S1-S4 each vary in their composition.

 When producing a 1x concentration, adjust by adding the same quantity of each.