Porducts
Can be used to synthesize all types of proteins
Protein Expression Kit
WEPRO7240 Core Kit
WEPRO7240 Core Kit
Users can freely alter the reaction scale according to their goals and the quantity of protein required.
Perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.
Features:
- Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.
- Target proteins can be confirmed through SDS-PAGE/CBB staining
Recommended for:
-
Users who require yields of soluble protein ranging from a few hundred micrograms to several miligrams.
-
Users who want to progress with the synthesis of dozens of samples as soluble protein simultaneously.
-
Users who want to perform screening.
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Users who want to perform crystal structure analysis.
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Users who want to perform interaction analysis.
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Users who want to perform function analysis.
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Users who want to perform complex formation (co-expression).
Flow of operations
Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.
Specification
Product Composition
Reagent Name |
Concentration |
Volume |
Quantity |
---|---|---|---|
WEPRO7240 |
240 OD |
1 mL |
1 |
SUB-AMIX SGC S1* | 40x |
600 µL |
1 |
SUB-AMIX SGC S2* |
40x |
600 µL |
1 |
SUB-AMIX SGC S3* | 40x |
600 µL |
1 |
SUB-AMIX SGC S4* | 40x |
600 µL |
1 |
Creatine Kinase |
20 mg/mL |
20 µL |
1 |
NTPmix |
25 mM |
120 µL |
1 |
5×Transcription Buffer LM |
5x |
240 µL |
1 |
RNase Inhibitor |
80K unit/mL |
15 µL |
1 |
SP6 RNA Polymerase |
80K unit/mL |
15 µL |
1 |
Storage Condition : -80 ℃
*SUB-AMIX SGC S1-S4 each vary in their composition.
When producing a 1x concentration, adjust by adding the same quantity of each.