Porducts
Can be used to synthesize all types of proteins
Premium PLUS Expression Kit
Recommended for first-time users and to synthesize protein on a small scale easily
Premium PLUS Expression Kit
Perfectly suited to confirm the expression of target proteins and synthesize protein on a small scale easily.
Features:
- No need to prepare reagents
- Reagents necessary for each reaction are already pre-packaged in tubes
- Synthesize proteins with just a few pipetting steps
- Target proteins can be confirmed through SDS-PAGE/CBB staining
- Contains enough for 8 reactions on a 226 µl reaction scale
Recommended for:
- Users who want to confirm the expression of target proteins easily using wheat germ cell-free expression system
- Users who are having difficulties with protein expression using other protein expression system
- Users who are having trouble because their target proteins are becoming insoluble
The reagents necessary for each reaction are pre-packaged into tubes, so there is no need to prepare any reagents.
Simply cut off the number of tubes per reaction you need, and you are ready to go.
Transcription reaction mixture (Transcription Premix LM)
Wheat germ extract (WEPRO9240)
Translation buffer (SUB-AMIX SGC)
Flow of operations
Specification
| Product Number | Product Name | Size | Instruction manual | Safety Data Sheet | |
|---|---|---|---|---|---|
| CFS-EDX-PLUS | Premium PLUS Expression Kit | 1 set |  |
|
Product Composition
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
pEU-E01-MCS |
1 µg/µL |
5 µL |
1 |
| pEU-E01-DHFR（Positive Control） | 1 µg/µL | 5 µL | 1 |
| PCR primer SPU | 1 µM | 100 µL | 1 |
| PCR primer deSP6E01 | 10 nM | 100 µL | 1 |
| WEPRO9240*1 | – | 10 µL | 8 |
| SUB-AMIX SGC | 1x | 206 µL | 8 |
| Transcription Premix LM *2 | 1x | 18 µL | 8 |
| Aluminum seal | – | – | 2 |
Storage Condition : -80 ℃
*1 : WEPRO9240 is based on WEPRO7240, with the addition of Creatine Kinase.
*2 : Transcription Premix LM is based on Transcription Buffer LM, with the addition of NTP Mix, SP6 RNA Polymerase and RNase Inhibitor.
