hGH ADA (human growth hormone) ELISA
CATALOG NUMBER : EL-141-878
The AffinityImmuno hGH ADA ELISA kit contains the components required for measuring antibodies to hGH in biological matrices such as human serum, plasma or cell culture supernatant.
INTRODUCTION
The AffinityImmuno hGH (Human Growth Hormone) ADA ELISA kit contains the components required for measuring antibodies to hGH in biological matrices such as human serum, plasma or cell culture supernatant.
PRINCIPLE OF THE ASSAY
This assay employs the indirect enzyme immunoassay technique. hGH is coated onto a 96 well microplate. Calibrator and test samples prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to hGH present in biological matrices are bound to the immobilized hGH. After washing away any unbound substances, antibodies
antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to hGH present in test samples. The color development is stopped and the intensity of the color is measured.
Product Specifications
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Application(s)
Measure anti-hGH antibodies -
Target Protein
hGH (human growth hormone) -
Alternative Name of Target
Somatropin -
Size
96 wells -
Precision
Intra-assay coefficient variation (CV) <20%, inter-assay CV <20% -
Detection Limit
30 ng/mL -
Storage Conditions
Stable at -20°C -
Safety Warnings and Precautions
Research Use Only. Follow instructions.
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EACH KIT INCLUDES :
– Coated microtiter plate
– 96 wells Calibrator diluent : 1.8ml
– Calibrator diluent (1 mg/mL) : 12ul
– 10X wash buffer : 50ml
– Assay buffer : 50ml
– 1000X detection reagent : 17ul
– TMB : 12ml
– TMB stop solution : 12ml
