GCSF ADA (granulocyte colony stimulating factor) ELISA | EL-141-73196
CATALOG NUMBER : EL-141-73196
The AffinityImmuno GCSF ADA ELISA kit contains the components required for measuring antibodies to GCSF in biological matrices such as human serum, plasma or cell culture supernatant.
INTRODUCTION
The AffinityImmuno GCSF ADA ELISA kit contains the components required for measuring antibodies to GCSF in biological matrices such as human serum, plasma or cell culture supernatant.
PRINCIPLE OF THE ASSAY
This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances,
antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color
development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.
Product Specifications
-
Application(s)
Measure anti-GCSF antibodies -
Target Protein
GCSF (granulocyte colony stimulating factor) -
Alternative Name of Target
Filgrastim -
Size
96 wells -
Precision
Intra-assay coefficient variation (CV) <20%, inter-assay CV <20% -
Detection Limit
45 ng/mL -
Storage Conditions
Stable at -20°C -
Safety Warnings and Precautions
Research Use Only. Follow instructions. -
Each Kit Includes
– Coated microtiter plate-96 wells
– Calibrator diluent 1.8 ml
– Calibrator diluent (1.15mg/mL -12 μl)
– 10X wash buffer-50 ml
– Assay buffer-50 ml
– 1000X detection reagent-17 μl
– TMB -12 ml
– TMB stop solution-12 ml
