GCSF-PEG ADA ELISA kit | EL-141-73196–GCSF-PEG-ADA
CATALOG NUMBER : EL-141-73196–GCSF-PEG-ADA
The AffinityImmuno GCSF-PEG ADA ELISA kit contains the components required for measuring antibodies to GCSF-PEG in biological matrices such as human serum, plasma or cell culture.
INTRODUCTION
The AffinityImmuno GCSF-PEG ADA ELISA kit contains the components required for measuring antibodies to GCSF-PEG in biological matrices such as human serum, plasma or cell culture.
PRINCIPLE OF THE ASSAY
This assay employs the indirect enzyme immunoassay technique. GCSF-PEG is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF-PEG present in biological matrices are bound to the immobilized GCSF-PEG. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development is proportional to the amount of antibodies to GCSF- PEG present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.
Product Specifications
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Catalog Number:
EL-141-73196–GCSF-PEG-ADA -
Application(s)
Measure anti-PEG-GCSF antibodies -
Target Protein
PEG-GCSF (Granulocyte colony -stimulating factor) -
Alternative Name of Target
PEG-filgrastim -
Gene ID
1440 -
Size
96 wells -
Target Specie(s)
Human -
Sensitivity
45 pg/mL -
Detection Method
Peroxidase/OD450 -
Storage Conditions
Stable at -20C for 1 year -
Safety Warnings and Precautions
Research Use Only. Follow instructions.
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EACH KIT INCLUDES :
– Coated microtiter plate
– 96 wells Calibrator diluent : 1.8ml
– Calibrator (250 ug/mL) : 12ul
– 10X wash buffer : 25ml
– Assay buffer : 50ml
– 1000X detection reagent : 17ul
– TMB : 12ml
– TMB stop solution : 12ml
– Plate sealers : 3
